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Microbiome associated with peri-implantitis versus periodontal health in individuals with a history of periodontal disease

机译：与牙周病历史有关的腹膜炎与牙周健康相关的微生物组

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The microbiome of peri-implantitis sites and healthy dental sites was determined in subjects with a history of periodontitis. Ten systemically healthy non-smokers received periodontal treatment before implant placement. Plaque samples were collected from four sites of one implant with peri-implantitis (bone loss ≥2mm; PPD ≥6mm; BOP/suppuration; 1year loading) and from one dental site per quadrant with periodontal health (PPD ≤3mm, CAL 4mm, absence of BOP and bone loss). Following DNA extraction, the bacteria present in each sample were determined by high-throughput sequencing of the V3-V4 region of the 16S-rRNA gene using the Illumina-MiSeq platform. OTUs were picked using QIIME. Differences between dental and implant sites were determined using linear discriminant analysis, effect size and diversity analyses were conducted using PAST v3.02. The microbiomes of diseased samples were less diverse than those found in health, although disease was associated with a higher abundance of taxa relative t...
机译：在具有牙周炎病史的受试者中确定了Peri-Implantitisitis和健康牙科部位的微生物组。在植入物放置前，十个系统健康的非吸烟者接受了牙周治疗。从一个植入物的四个位点收集斑块样品（骨骼损失≥2mm; ppd≥6mm; bop / pupporting;> 1年装载），并且每象限的一个牙科部位（ppd≤3mm，cal <4mm不存在BOP和骨质损失）。在DNA提取之后，通过使用Illumina-MiSeq平台的16S-RRNA基因的V3-V4区的高通量测序确定每个样品中存在的细菌。奥斯·奥斯使用豆。使用线性判别分析测定牙科和植入部位之间的差异，使用过去的V3.02进行效果大小和分化分析。患病样品的微生物比在健康中发现的微生物均不等，尽管疾病与较高的分类群相对......

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