• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oral Microbiology >Investigation of fitness cost, copy number variation and excision rate of Tn916/Tn916-like elements in oral streptococci and enterococci
【24h】

Investigation of fitness cost, copy number variation and excision rate of Tn916/Tn916-like elements in oral streptococci and enterococci

机译：在口腔链球菌和肠球菌中的健身成本，拷贝数变异和TN916 / TN916样元素的切除率

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Tn916 and other related elements are broad host range conjugative transposons that are widely spread in bacteria. These elements contribute to the spread of antibiotic resistance genes (ARG) between bacterial species. Variation in the Tn916/Tn916-like elements copy number (Tn916CN) and their excision frequency (Tn916ExFr) from their host genome could play a role in propagation of ARG. In this study, the Tn916CN and the Tn916ExFr in oral streptococci and enterococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the fitness cost associated with new acquisition of Tn916 in a selected oral streptococci and enterococci strains. Amelioration of fitness cost and mechanism(s) to mitigate fitness loss were evaluated by ddPCR and next generation sequencing (NGS). We found the ddPCR is superior to southern blot to determine Tn916CN. It was also found that both the excision rate of Tn916 and its autonomous replication increase because of antimicrobial challenge. Loss of b...
机译：TN916和其他相关元素是广泛的宿主范围缀合转座子，其在细菌中广泛传播。这些元素有助于细菌物种之间的抗生素抗性基因（Arg）的扩散。 TN916 / TN916样元素拷贝数（TN916CN）及其宿主基因组的常量（TN916CN）的变化可以在arg传播中发挥作用。在该研究中，使用液滴数量PCR（DDPCR）测定口服链球菌和肠球菌中的TN916CN和TN916EXFR。此外，我们研究了在选定的口服链球菌和肠球菌菌株中对新收购TN916相关的健康成本。通过DDPCR和下一代测序（NGS）评估健身成本和减轻健身损失的机制的改善。我们发现DDPCR优于南方印迹，以确定TN916CN。还发现，由于抗微生物挑战，TN916的切除率及其自主复制增加。失去B ...

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号