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首页> 外文期刊>Journal of Zhejiang University. Science, B >Influence of injection of Chinese botulinum toxin type A on the histomorphology and myosin heavy chain composition of rat gastrocnemius muscles
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Influence of injection of Chinese botulinum toxin type A on the histomorphology and myosin heavy chain composition of rat gastrocnemius muscles

机译：中国肉毒杆菌毒素型A对大鼠胃肠肌肌肉组织和肌球蛋白重链组成的影响

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摘要

Background and objective: botulinum toxin type A (BoNT/A) is a metalloprotease that blocks synaptic transmission via the cleavage of a synaptosomal-associated protein of 25 kDa (SNAP-25). It has gained widespread use as a treatment for cerebral palsy and skeletal muscle hypertrophy. In China, Chinese botulinum toxin type A (CBTX-A), a type of BoNT/A, is in widespread clinical use. However, the changes in the morphological and biochemical properties of treated muscles and in remote muscles from the CBTX-A injection site are relatively unknown. Therefore, we investigated the changes in histomorphology and myosin heavy chain (MyHC) isoform composition and distribution in rat gastrocnemius muscles after intramuscular injection of CBTX-A. Methods: The weakness of the injected muscles was assessed periodically to identify their functional deficiency. Muscle slices were stained with hematoxylin-eosin (HE) and adenosine triphosphatase (ATPase). MyHC isoform composition was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to uncover changes in morphological and biochemical properties. Results: Our findings demonstrate that following injection of CBTX-A 5 U into rat gastrocnemius muscles, shifts in MyHC isoform composition emerged on the third day after injection and peaked in the fourth week. The composition remained distinctly different from that of the control group after the twelfth week. More specifically, there was a decrease in the proportion of the type IIb isoform and an increase in the proportions of type IIx, type IIa, and type I isoforms. Conclusions: Data revealed that CBTX-A led to a shift in MyHC composition towards slower isoforms and that the MyHC composition remained far from normal six months after a single injection. However, no noticeable remote muscle weakness was induced.
机译：背景和目的：肉毒杆菌毒素A（Bont / A）是一种金属蛋白酶，其通过25kDa（Snap-25）的突触体相关蛋白的切割阻断突触传递。它越来越广泛用作脑瘫和骨骼肌肥大的治疗方法。在中国，中国肉毒杆菌毒素类型A（CBTX-A），一种突发/ A，是在广泛的临床用途中。然而，来自CBTX-A注射部位的处理肌肉和远程肌肉的形态学和生化特性的变化相对未知。因此，我们研究了在肌内注射CBTX-a的肌肉内注射后大鼠胃肠杆菌肌肉中的组织组态和肌球蛋白重链（MYHC）的变化。方法：定期评估注入肌肉的弱点以确定其功能缺陷。用苏木精 - 曙红（He）和腺苷三磷酸酶（ATP酶）染色肌肉切片。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS-PAGE）分析MYHC同种型组合物，以发现形态学和生化特性的变化。结果：我们的研究结果表明，在将CBTX-A 5 u注射到大鼠胃肠肌中，在注射后第三天出现的MyHC同种型组合物的变化，并在第四周达到峰值。组合物在第十二周后与对照组明显不同。更具体地，IIB类同种型的比例减少，型IIX型比例的增加，IIA型和I型同种型。结论：数据显示，CBTX-A导致MyHC组合的转变为较慢的同种型，并且MyHC组成留在一次注射后六个月的正常六个月。但是，没有引起明显的远程肌肉弱点。

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