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首页> 外文期刊>Investigative ophthalmology & visual science >Long-term changes in myosin heavy chain composition after botulinum toxin a injection into rat medial rectus muscle.
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Long-term changes in myosin heavy chain composition after botulinum toxin a injection into rat medial rectus muscle.

机译：肉毒杆菌毒素注射到大鼠内侧直肌后肌球蛋白重链组成的长期变化。

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摘要

PURPOSE: To study long-term changes of extraocular muscles after botulinum toxin (Botx) A-induced paralysis, with special emphasis on myosin heavy chain (MyHC) isoform pattern in muscle fibers. METHODS: Botx A (5 IU) was injected into the ocular medial rectus (MR) muscles of adult rats. After 1, 5, and 8 months muscle cross sections were examined immunohistochemically, histochemically, and morphometrically. MyHC content was analyzed by gel electrophoresis. RESULTS: Paralyzed MR muscles displayed mildly atrophic and hypertrophic muscle fibers and decreased oxidative metabolism, due to decreased succinate dehydrogenase activity. However, muscle morphology was not grossly disturbed. MyHC profile was shifted toward slower isoforms. Electrophoretic analysis showed that the share of MyHCI, and especially of MyHCIIa and MyHCIIx/d, increased several fold, whereas the share of MyHCIIb decreased heavily during the first 5 months. Immunohistochemical analysis generally mirrored the results obtained by electrophoresis. Moreover, specific extraocular MyHC isoform MyHCeom disappeared and could not be detected during the whole experimental period. The portion of MyHCIIb relatively increased 8 months after Botx A injection, although the MyHC profile was still far from normal. CONCLUSIONS: These long-lasting changes in Botx A-paralyzed ocular MR muscles most probably reflect their inability to regain their unique functional characteristics after new motor end plate formation and recovery of muscle contraction.
机译：目的：研究肉毒杆菌毒素（Botx）A引起的麻痹后眼外肌的长期变化，特别强调肌纤维中的肌球蛋白重链（MyHC）亚型。方法：将Botx A（5 IU）注射到成年大鼠的眼内直肌（MR）中。在1、5和8个月后，通过免疫组织化学，组织化学和形态计量学检查肌肉横截面。通过凝胶电泳分析MyHC含量。结果：由于琥珀酸脱氢酶活性降低，瘫痪的MR肌肉显示出轻度萎缩和肥大的肌肉纤维，氧化代谢降低。但是，肌肉形态并未受到严重干扰。 MyHC档案已转向较慢的亚型。电泳分析表明，MyHCI的份额，尤其是MyHCIIa和MyHCIIx / d，增加了几倍，而MyHCIIb的份额在头5个月中急剧下降。免疫组织化学分析通常反映出电泳获得的结果。此外，在整个实验期间，特定的眼外MyHC亚型MyHCeom消失了，无法检测到。注射Botx A后8个月，MyHCIIb的比例相对增加，尽管MyHC概况仍远未达到正常水平。结论：Botx A瘫痪的眼部MR肌肉的这些持久变化很可能反映了它们在新的运动终板形成和肌肉收缩恢复后无法恢复其独特的功能特征。

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