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首页> 外文期刊>RSC Advances >Antibody-free colorimetric detection of chlorothalonil in cucumber via the inhibition of an enzyme-triggered reaction
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Antibody-free colorimetric detection of chlorothalonil in cucumber via the inhibition of an enzyme-triggered reaction

机译：通过抑制酶触发反应的黄瓜中氯洛尼尼洛洛尼洛尼洛尼洛的无抗体比色测量

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Currently known rapid determination of fungicides usually relies on antibody-based immunoassay. This paper reports a simple antibody-free colorimetric assay for chlorothalonil via the inhibition of an enzyme-triggered reaction. The enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase was significantly inhibited by chlorothalonil, and the color change of NBT-PMS system induced from NADH formation was suppressed, which could be used indirectly to assay chlorothalonil. The limit of detection (LOD) was 0.05 μM with a linear range from 0.5 to 10 μM, and the detection of 1 μM chlorothalonil in solution was achieved with a naked-eye readout. In addition, the colorimetric measurement results of the cucumber samples showed a good recovery rate, although the sensitivity was less effective than the instrumental method. Nevertheless, the results demonstrates that the chlorometric method provides potential opportunities for reliable, cost-effective quantitative detection for chlorothalonil residues in vegetables.
机译：目前已知的杀菌剂的快速测定通常依赖于基于抗体的免疫测定。本文通过抑制酶触发反应，报道了氯洛尼尼洛尼洛尼洛尼尼的无抗体比色测定。氯洛尼尼洛尼洛尼洛尼洛尼醛抑制甘氨醛-3-磷酸脱氢酶的酶活性，抑制了NADH形成诱导的NBT-PMS系统的颜色变化，其可间接地用于测定氯洛尼尼尔。检测极限（LOD）为0.05μm，线性范围为0.5至10μm，通过裸眼读数达到溶液中1μm氯洛尼洛的检测。此外，黄瓜样品的比色测量结果显示出良好的回收率，尽管灵敏度比乐器方法较低。然而，结果表明，氯测定方法为蔬菜中氯含量残留物的可靠，经济有效的定量检测提供了潜在的机会。

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