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首页> 外文期刊>RSC Advances >Chemical composition, nutraceuticals characterization, NMR confirmation of squalene and antioxidant activities of Basella rubra L. seed oil
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Chemical composition, nutraceuticals characterization, NMR confirmation of squalene and antioxidant activities of Basella rubra L. seed oil

机译：化学成分，营养保健品表征，核苷酸核苷酸和Basella Rubra L.种子油的抗氧化活性

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Basella rubra (Malabar spinach) is a commonly consumed green leafy vegetable in southern parts of India. The chemical composition, nutraceuticals characterization, squalene Nuclear Magnetic Resonance (NMR), in vitro antioxidant activities and cytotoxicity of B. rubra seed oil (33.08%) was investigated. Gas chromatography-mass spectrometry (GC/MS) analysis revealed the presence of palmitic (27.21 μmol%), oleic (33.83 μmol%) and linoleic acid (26.02 μmol%) with a total of 64.38 μmol% unsaturated fatty acids respectively. HPLC nutraceutical characterization showed a major constituent of gallic acid (11.23 mg%), γ-tocopherols (17.74 mg%), cycloartenylferulate (1.7 mg%), and squalene (1 g%). Squalene was further recovered (98%), purified (99.9%), and confirmed through ~(1) H and ~(13) C NMR. The in vitro antioxidant activities recorded by using 2,2-diphenyl-1-picrylhydrazyl (EC _(50) = 6 mg mL ~(?1) ), ferric reducing antioxidant power (361.85 mM of Trolox Eq./100 g) and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (EC _(50) = 56.19 mg mL ~(?1) ) scavenging activity. In vitro cytotoxicity assessed on 3T3-L1 showed good cell survival without any toxicity (upto 400 μg mL ~(?1) ). B. rubra seed oil has proven nutraceuticals and antioxidant potentials with least toxicity which can be recommended for functional foods applications.
机译：Basella Rubra（Malabar Spinach）是印度南部的常用绿叶蔬菜。研究了化学成分，营养素表征，角鲨烯核磁共振（NMR），体外抗氧化活性和B. rubra种子油（33.08％）的细胞毒性。气相色谱 - 质谱（GC / MS）分析显示出棕榈酸（27.21μmol％），油酸（33.83μmol％）和亚油酸（26.02μmol％）的存在，分别具有64.38μmol％的不饱和脂肪酸。 HPLC Nutraceutical表征显示了Gallic酸（11.23mg％），γ-生育酚（17.74mg％），环戊烯键（1.7mg％）和squalene（1g％）的主要组成部分。将Squalene进一步回收（98％），纯化（99.9％），并通过〜（1）H和〜（13）C NMR。通过使用2,2-二苯基-1-富铬酰基（EC _（50）= 6mg ml〜（α1）），减少抗氧化能力（361.85mm的Trolox Eq./100g）和抗氧化剂（EC _（50）= 6mg）和2,2'-氮杂-PIS-3-乙基异噻唑啉-6-磺酸（EC _（50）= 56.19mg mL〜（α1））清除活性。在3T3-L1上评估的体外细胞毒性显示出良好的细胞存活，没有任何毒性（高达400μgml〜（α1））。 B. Rubra Seed油已证明具有最小毒性的营养保存和抗氧化潜力，这可能推荐用于功能性食品应用。

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