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Enrichment of nitrifying bacteria and microbial diversity analysis by high-throughput sequencing

机译:通过高通量测序富化硝化细菌和微生物分析分析的富集

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To achieve nitrifying bacteria (ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB)) growth, activated sludge from the A ~(2) /O process in a wastewater treatment plant (WWTP) was used as seed sludge to guide the industrial culture of nitrifying bacteria for ammonia-contaminated wastewater treatment. The bacterial community was enriched in a fully automatic miniature fermenter by an alternating mode of intermittent operation mode in the daytime and a continuous ammonia feeding mode for intermittent operation during the night. The results indicated that the nitrifying bacteria community can be enriched by this alternating operation mode, resulting in a maximum ammonia oxidation rate reaching approximately 110 mg L ~(?1) h ~(?1) and a nitrite oxidation rate of approximately 50 mg L ~(?1) h ~(?1) . High-throughput sequencing demonstrated that Nitrosomonas and Nitrospira belonging to AOB and NOB, respectively, were predominant in the activated sludge after 25 days at a ratio extending from 2.94% to 60.66%.
机译:为了实现硝化细菌(氨氧化细菌(AOB)和亚硝酸盐 - 氧化细菌(NOB))生长,从废水处理厂(WWTP)中的A〜(2)/ O过程中的活性污泥用作导液硝化细菌的产业培养氨污染废水处理。通过白天的间歇操作模式的交替模式和连续的氨馈电模式,在夜间的连续氨馈电模式中富含细菌群体富含全自动微型发酵罐。结果表明,硝化细菌群体可以通过这种交替的操作模式来富集,导致最大氨氧化速率达到约110mg1〜(α1)H〜(α1)和约50mg的亚硝酸盐氧化速率〜(?1)H〜(?1)。高通量测序证明,亚硝基菌和氮气分别属于AOB和NOB,在25天后在25天后的活性污泥中占主导地位,其比例从2.94%延伸至60.66%。

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