A fully automatic miniature fermenter was operated to enrich ammoniaoxidizing bacteria (AOB) using the activated sludge obtained from the A2/O process in a wastewater treatment plant (WWTP) as the seed sludge. The results revealed that the AOB community can be enriched by a continuous ammonia feeding mode for intermittent operation, resulting in the appropriate regulation of free ammonia (FA) and free nitrous acid (FNA) levels. The maximum ammonia oxidation rate was over 170 mg/ (L·h) and nitrite accumulation ratio over 80%. High-throughput sequencing showed that Nitrosomonas belonging to AOB, were highly enriched after 27 days, having increased from 0.24% to 53.03%.
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