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首页> 外文期刊>BMC Microbiology >Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli
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Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli

机译：外源多不饱和脂肪酸（PUFA）促进大肠杆菌中生长，磷脂组合物，膜渗透性和毒力表型的变化

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The utilization of exogenous fatty acids by Gram-negative bacteria has been linked to many cellular processes, including fatty acid oxidation for metabolic gain, assimilation into membrane phospholipids, and control of phenotypes associated with virulence. The expanded fatty acid handling capabilities have been demonstrated in several bacteria of medical importance; however, a survey of the polyunsaturated fatty acid responses in the model organism Escherichia coli has not been performed. The current study examined the impacts of exogenous fatty acids on E. coli. All PUFAs elicited higher overall growth, with several fatty acids supporting growth as sole carbon sources. Most PUFAs were incorporated into membrane phospholipids as determined by Ultra performance liquid chromatography-mass spectrometry, whereas membrane permeability was variably affected as measured by two separate dye uptake assays. Biofilm formation, swimming motility and antimicrobial peptide resistance were altered in the presence of PUFAs, with arachidonic and docosahexaenoic acids eliciting strong alteration to these phenotypes. The findings herein add E. coli to the growing list of Gram-negative bacteria with broader capabilities for utilizing and responding to exogenous fatty acids. Understanding bacterial responses to PUFAs may lead to microbial behavioral control regimens for disease prevention.
机译：通过革兰氏阴性细菌利用外源性脂肪酸已经连接到许多细胞方法，包括用于代谢增益的脂肪酸氧化，分化为薄膜磷脂，以及控制与毒力相关的表型。在医学重要性的几种细菌中已经证明了扩增的脂肪酸处理能力;然而，尚未进行对模型生物体大肠杆菌中的多不饱和脂肪酸反应的调查。目前的研究检测了外源脂肪酸对大肠杆菌的影响。所有PUFA都引发了更高的整体生长，几种脂肪酸支持生长作为唯一的碳源。大多数PUFA被掺入膜磷脂，如超级性能液相色谱 - 质谱法测定的，而通过两个单独的染料摄取测定测量，膜渗透性可变地受到可变的影响。生物膜形成，游泳运动和抗微生物肽抗性在Pufas存在下改变，用花生和十二碳六烯酸引发对这些表型的强烈改变。本文的发现将大肠杆菌加入到革兰氏阴性细菌的生长列表中，具有更广泛的能力，用于利用和应对外源脂肪酸。了解对PUFA的细菌反应可能导致疾病预防的微生物行为控制方案。

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