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首页> 外文期刊>Frontiers in Public Health >The Phenotypic and Transcriptomic Response of the Caenorhabditis elegans Nematode to Background and Below-Background Radiation Levels
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The Phenotypic and Transcriptomic Response of the Caenorhabditis elegans Nematode to Background and Below-Background Radiation Levels

机译：Caenorhabdisegiss Nematode对背景和低于背景辐射水平的表型和转录组反应

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Studies of the biological effects of low-level and below-background radiation are important in understanding the potential effects of radiation exposure in humans. To study this issue we exposed the nematode Caenorhabditis elegans to average background and below-background radiation levels. Two experiments were carried-out in the underground radiation biology laboratory at the Waste Isolation Pilot Plant (WIPP) in New Mexico USA. The first experiment used na?ve nematodes with data collected within 1 week of being placed underground. The second experiment used worms that were incubated for 8 months underground at below background radiation levels. Nematode eggs were placed in two incubators, one at low radiation (ca.15.6 nGy/hr) and one supplemented with 2 kg of natural KCl (ca. 67.4 nGy/hr). Phenotypic variables measured were: (1) egg hatching success (2) body size from larval development to adulthood, (3) developmental time from egg to egg laying adult, and (4) egg laying rate of young adult worms. Transcriptome analysis was performed on the first experiment on 72 h old adult worms. Within 72 h of being underground, there was a trend of increased egg-laying rate in the below-background radiation treatment. This trend became statistically significant in the group of worms exposed to below-background radiation for 8 months. Worms raised for 8 months in these shielded conditions also had significantly faster growth rates during larval development. Transcriptome analyses of 72-h old na?ve nematode RNA showed significant differential expression of genes coding for sperm-related proteins and collagen production. In the below-background radiation group, the genes for major sperm protein ( msp , 42% of total genes) and sperm-related proteins (7.5%) represented 49.5% of the total genes significantly up-regulated, while the majority of down-regulated genes were collagen ( col , 37%) or cuticle-related (28%) genes. RT-qPCR analysis of target genes confirmed transcriptomic data. These results demonstrate that exposure to below-background radiation rapidly induces phenotypic and transcriptomic changes in C. elegans within 72 h of being brought underground.
机译：对低水平和低于背景辐射的生物学效应的研究对于了解人类辐射暴露的潜在影响很重要。为了研究这个问题，我们将Nematode Caenorhabditis elegans暴露于平均背景和后面的背景辐射水平。在新墨西哥州美国废物隔离试验厂（WIPP）的地下辐射生物实验室中进行了两次实验。第一个实验使用Na ve Nematodes，其中包含在地下1周内收集的数据。第二个实验使用蠕虫在低于背景辐射水平的地下孵育8个月。 NEMATODE卵置于两个培养箱中，一个在低辐射（CA.15.6 NGY / HR）中，一个含有2千克天然KCl（约67.4吨/小时）。测量的表型变量是：（1）卵孵化成功（2）幼虫发育的体型，（3）从鸡蛋到鸡蛋铺设成人的发育时间，和（4）鸡蛋敷料的年轻成年蠕虫。转录组分析在72 H老成年蠕虫上进行第一次实验进行。在地下72小时内，在下面的背景辐射治疗中存在较大的鸡蛋率提高。这种趋势在暴露于背景下辐射8个月的蠕虫中的统计学意义。在这些屏蔽条件下持续8个月的蠕虫在幼虫发育过程中也具有明显更快的增长率。 72-H旧的Naαve Nematode RNA的转录组分析表明，编码精子相关蛋白和胶原蛋白的基因的显着差异表达。在下面的背景辐射组中，主要精子蛋白（MSP，总基因42％）和精子相关蛋白质（7.5％）的基因表示49.5％的总基因显着上调，而大多数下降 - 调节基因是胶原（Col，37％）或相关的（28％）基因。靶基因的RT-QPCR分析证实了转录组数据。这些结果表明，暴露于背景辐射的暴露迅速诱导在地下72小时内释放C.埃贝罗斯的表型和转录组变化。

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