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首页> 外文期刊>Applied Microbiology >Antibiofilm Activity of Lactobacillus plantarum 12 Exopolysaccharides against Shigella flexneri
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Antibiofilm Activity of Lactobacillus plantarum 12 Exopolysaccharides against Shigella flexneri

机译：乳酸杆菌的抗生素12克里尔·柔屈菌的外核蛋白酶活性

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In developing countries, Shigella flexneri is the most common enteric pathogen causing bacillary dysentery. Biofilm formation by S. flexneri can cause the emergence of antibiotic-resistant strains, which poses serious threats to food safety and human health. In this study, the effects of Lactobacillus plantarum 12 exopolysaccharides (L-EPSs) and S. flexneri exopolysaccharides (S-EPSs) on S. flexneri CMCC51574 biofilm formation were investigated. The results showed that L-EPS could decrease polysaccharide production in the extracellular polymeric matrix of S. flexneri and inhibit biofilm formation by S. flexneri . L-EPS could decrease the minimum biofilm elimination concentration (MBEC) of antibiotics against S. flexneri biofilm and inhibit S. flexneri adhesion to and invasion into HT-29 cell monolayers, which might be ascribed to S. flexneri biofilm disturbance by L-EPS. In contrast, S-EPS exhibited the opposite effects compared to L-EPS. The monosaccharide composition analysis showed that L-EPS was composed of mannose, glucuronic acid, galactosamine, glucose, galactose, and xylose, with the molar ratio of 32.26:0.99:1.79:5.63:0.05:4.07, while S-EPS was composed of mannose, glucuronic acid, galactosamine, glucose, and galactose, with the molar ratio of 25.43:2.28:7.13:5.35. L-EPS was separated into the neutral polysaccharide L-EPS 1-1 and the acidic polysaccharide L-EPS 2-1 by ion-exchange chromatography and gel chromatography. L-EPS 2-1 exerted higher antibiofilm activity than L-EPS 1-1. The antibiofilm activity of L-EPS might be associated with its structure.IMPORTANCE S. flexneri is a widespread foodborne pathogen causing food contamination and responsible for food poisoning outbreaks related to various foods in developing countries. Not only has biofilm formation by S. flexneri been difficult to eliminate, but it has also increased the drug resistance of the strain. In the present study, it was demonstrated that L-EPSs secreted by Lactobacillus plantrum 12 could inhibit S. flexneri biofilm formation on, adhesion to, and invasion into HT-29 cells. Also, L-EPSs could decrease the minimum biofilm elimination concentration (MBEC) of the antibiotics used against S. flexneri biofilm. Therefore, L-EPSs were shown to be bioactive macromolecules with the potential ability to act against S. flexneri infections.
机译：在发展中国家，Shigella Flexneri是最常见的肠溶病病原体，导致人霉菌痢疾。由S.Flexeri的生物膜形成可导致抗生素抗性菌株的出现，这给食品安全和人类健康带来了严重的威胁。在这项研究中，研究了乳酸杆菌12次蛋白酶（L-EPS）和S.Flexeri ukcy多糖（S-EPS）对S.Flexeri CMCC51574生物膜形成的影响。结果表明，L-EP可以降低S.Flexeri细胞外聚合物基质中的多糖产生并通过S.Flexeri抑制生物膜形成。 L-EP可以降低抗生素的抗生素的最低生物膜消除浓度（MBEC），并抑制S.Flexeri粘附和侵袭HT-29细胞单层，这可能由L-EPS归因于S.Flexneri生物膜干扰。相比之下，与L-EPS相比，S-EP表现出相反的效果。单糖组成分析显示L-EP由甘露糖，葡糖醛酸，半乳糖胺，葡萄糖，半乳糖和木糖组成，摩尔比为32.26：0.99：1.79：5.63：0.05：4.07，而S-EP组成甘露糖，葡糖醛酸，半乳糖胺，葡萄糖和半乳糖，摩尔比为25.43：2.28：7.13：5.35。通过离子交换色谱法和凝胶色谱法将L-EP分离成中性多糖L-EPS 1-1和酸性多糖L-EPS 2-1。 L-EPS 2-1比L-EPS 1-1施加更高的抗抗菌活动。 L-EPS的抗生素活性可能与其结构相关。分析S. Flexneri是一种普遍的食源性病原体，导致食物污染，并负责与发展中国家的各种食物有关的食物中毒爆发。不仅通过S. Flexeri难以消除生物膜形成，而且还增加了菌株的耐药性。在本研究中，证明由乳杆菌12分泌的L-EPS可以抑制S.Flexeri Biofilm的形成，粘附和侵入HT-29细胞。此外，L-EPS可以降低针对S.Flexneri生物膜使用的抗生素的最小生物膜消除浓度（MBEC）。因此，L-EPS被证明是生物活性大分子，具有对S. Flexni感染的潜在能力。

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