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首页> 外文期刊>Applied Microbiology >Heat-Treated Virus Inactivation Rate Depends Strongly on Treatment Procedure: Illustration with SARS-CoV-2
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Heat-Treated Virus Inactivation Rate Depends Strongly on Treatment Procedure: Illustration with SARS-CoV-2

机译：热处理的病毒失活率在治疗程序中强烈取决于：与SARS-COV-2的插图

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Decontamination helps limit environmental transmission of infectious agents. It is required for the safe reuse of contaminated medical, laboratory, and personal protective equipment, and for the safe handling of biological samples. Heat treatment is a common decontamination method, notably used for viruses. We show that for liquid specimens (here, solution of SARS-CoV-2 in cell culture medium), the virus inactivation rate under heat treatment at 70°C can vary by almost two orders of magnitude depending on the treatment procedure, from a half-life of 0.86?min (95% credible interval [CI] 0.09, 1.77) in closed vials in a heat block to 37.04?min (95% CI 12.64, 869.82) in uncovered plates in a dry oven. These findings suggest a critical role of evaporation in virus inactivation via dry heat. Placing samples in open or uncovered containers may dramatically reduce the speed and efficacy of heat treatment for virus inactivation. Given these findings, we reviewed the literature on temperature-dependent coronavirus stability and found that specimen container types, along with whether they are closed, covered, or uncovered, are rarely reported in the scientific literature. Heat-treatment procedures must be fully specified when reporting experimental studies to facilitate result interpretation and reproducibility, and must be carefully considered when developing decontamination guidelines.IMPORTANCE Heat is a powerful weapon against most infectious agents. It is widely used for decontamination of medical, laboratory, and personal protective equipment, and for biological samples. There are many methods of heat treatment, and methodological details can affect speed and efficacy of decontamination. We applied four different heat-treatment procedures to liquid specimens containing SARS-CoV-2. Our results show that the container used to store specimens during decontamination can substantially affect inactivation rate; for a given initial level of contamination, decontamination time can vary from a few minutes in closed vials to several hours in uncovered plates. Reviewing the literature, we found that container choices and heat treatment methods are only rarely reported explicitly in methods sections. Our study shows that careful consideration of heat-treatment procedure—in particular the choice of specimen container and whether it is covered—can make results more consistent across studies, improve decontamination practice, and provide insight into the mechanisms of virus inactivation.
机译：净化有助于限制传染病的环境传播。需要安全再利用受污染的医疗，实验室和个人防护装备，并用于安全处理生物样品。热处理是一种常见的去污方法，特别是用于病毒。我们表明，对于液体标本（这里，SARS-COV-2在细胞培养基中的溶液），在70℃下热处理的病毒失活率可以根据处理程序，从一半的处理过程中的几乎不等。 - 在热嵌段中的封闭小瓶中（95％可靠间隔[CI] 0.09,1.77）的生物（95％可信的间隔[CI] 0.09,1.77），在干燥烘箱中的未覆盖板中（95％CI 12.64,869.82）。这些发现表明蒸发在病毒失活的关键作用通过干热灭活。在开放或未覆盖的容器中放置样品可能会显着降低热处理灭活的热处理的速度和功效。鉴于这些发现，我们在温度依赖性冠状病毒稳定性上审查了文献，发现样本容器类型以及它们是否被封闭，覆盖或未覆盖，很少报道在科学文献中。报告实验研究时，必须完全指定热处理程序，以促进结果解释和可重复性，并且在开发净化指南时必须仔细考虑。热量是一种针对大多数传染病的强大武器。它广泛用于医疗，实验室和个人防护设备和生物样品的净化。有许多热处理方法，方法细节会影响去污的速度和功效。我们将四种不同的热处理程序应用于含有SARS-COV-2的液体样本。我们的研究结果表明，用于在去污期间储存标本的容器可以显着影响失活率;对于给定的初始污染水平，去污时间可以在未封闭的小瓶中几分钟内变化到未覆盖板中的几个小时。审查文献，我们发现集装箱选择和热处理方法仅在方法部分中明确报道。我们的研究表明，仔细考虑热处理程序 - 特别是样本容器的选择以及是否覆盖 - 可以在研究中更加一致，改善去污做法，并提供对病毒失活机制的洞察力。

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