• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Animal Nutrition >Gut microbiota absence and transplantation affect growth and intestinal functions: An investigation in a germ-free pig model
【24h】

Gut microbiota absence and transplantation affect growth and intestinal functions: An investigation in a germ-free pig model

机译：肠道微生物瘤缺失和移植影响生长和肠功能：在无菌猪模型中进行调查

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study was conducted to investigate host–microbiota interactions and explore the effects of maternal gut microbiota transplantation on the growth and intestinal functions of newborns in a germ-free (GF) pig model. Twelve hysterectomy-derived GF Bama piglets were reared in 6 sterile isolators. Among them, 6 were considered as the GF group, and the other 6 were orally inoculated with healthy sow fecal suspension as fecal microbiota transplanted (FMT) group. Another 6 piglets from natural birth were regarded as the conventional (CV) group. The GF and FMT groups were hand-fed with Co60-γ-irradiated sterile milk powder, while the CV group was reared by lactating Bama sows. All groups were fed for 21?days. Then, all piglets and then were switched to sterile feed for another 21 days. Results showed that the growth performance, nutrient digestibility, and concentrations of short-chain fatty acids in the GF group decreased ( P ?0.05). Meanwhile, the serum urea nitrogen concentration and digesta pH values in the GF group increased compared with those in the FMT and CV groups ( P ?0.05). Compared with the CV?group, the GF group demonstrated upregulation in the mRNA expression levels of intestinal barrier function-related genes in the small intestine ( P ?0.05). In addition, the mRNA abundances of intestinal development and absorption-related genes in the small intestine and colon were higher in the GF group than in the CV and FMT groups ( P ?0.05). The FMT group exhibited greater growth performance, lipase activity, and nutrient digestibility ( P ?0.05), higher mRNA expression levels of intestinal development and barrier-related genes in the small intestine ( P ?0.05), and lower mRNA abundances of pro-inflammatory factor in the colon and jejunum ( P ?0.05) than the CV group. In conclusion, the absence of gut microbes impaired the growth and nutrient digestibility, and healthy sow gut microbiota transplantation increased the growth and nutrient digestibility and improved the intestinal development and barrier function of newborn piglets, indicating the importance of intestinal microbes for intestinal development and functions.
机译：进行该研究以研究宿主微生物A相互作用，并探讨母体肠道微生物会移植对无菌（GF）猪模型中新生儿生长和肠功能的影响。 12个无菌隔离器中饲养12个子宫切除术衍生的GF BAMA仔猪。其中，6被认为是GF组，另外6种，用健康的母猪悬浮液作为粪便微生物移植（FMT）组口服接种。来自天然生出生的另外6只仔猪被认为是常规（CV）组。通过CO60-γ-辐照的无菌奶粉进行GF和FMT组，而CV基团通过哺乳母猪母猪饲养。所有群体都被喂21天。然后，所有仔猪然后切换到无菌进料21天。结果表明，GF组中短链脂肪酸的生长性能，营养消化率和浓度降低（P <0.05）。同时，与FMT和CV基团中的那些相比，GF组中的血清尿素氮浓度和Digesta pH值增加（P <0.05）。与CVα组相比，GF组在小肠中的肠道阻隔函数相关基因的MRNA表达水平上表现出上调（P <0.05）。此外，GF组在小肠和结肠中的肠道发育和吸收相关基因的mRNA丰度高于CV和FMT基团（P <0.05）。 FMT组表现出更大的生长性能，脂肪酶活性和营养消化率（P＆Δ05），小肠中肠道发育和屏障相关基因的较高mRNA表达水平（P <0.05），以及降低mRNA丰富比CV组结肠和Jejunum的促炎症因子（P <0.05）。总之，肠道微生物的缺乏损害了生长和营养消化率，健康母猪肠道微生物液移植增加了生长和养分消化率，并改善了新生仔猪的肠道发育和障碍功能，表明肠道微生物对肠道发育和功能的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号