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首页> 外文期刊>Journal of clinical laboratory analysis. >Performance evaluation of leukocyte differential on the hematology analyzer Celltac G compared with two hematology analyzers, reference flow cytometry method, and two manual methods
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Performance evaluation of leukocyte differential on the hematology analyzer Celltac G compared with two hematology analyzers, reference flow cytometry method, and two manual methods

机译：白细胞差异对血液学分析仪Celltac G的性能评价与两种血液学分析仪，参考流式细胞术法和两种手动方法

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Background The automated hematology analyzer Celltac G (Nihon Kohden) was designed to improve leukocyte differential performance. Comparison with analyzers using different leukocyte detection principles and differential leukocyte count on wedge film (Wedge-Diff) shows its clinical utility, and comparison with immunophenotypic leukocyte differential reference method (FCM-Ref) shows its accuracy performance. Methods For method comparison, 598 clinical samples and 46 healthy volunteer samples were selected. The two comparative hematology analyzers (CAAs) used were XN-9000 (Sysmex) and CELL-DYN Sapphire (Abbott). The FCM-Ref provided by the Japanese Society for Laboratory Hematology was selected, and a flow cytometer Navios (Beckman-Coulter) was used. In manual differential, two kinds of automated slide makers were used: SP-10 (Sysmex) for wedge technique and SPINNER-2000 (Lion-Power) for spinner technique. The spinner technique avoids the issue of Wedge-Diff smudge cells by removing the risk of breaking cells and non-uniformity of blood cell distribution on films (Spinner-Diff). Results The Celltac G showed sufficient comparability ( r =?0.67–1.00) with the CAAs for each leukocyte differential counting value at 0.00–40.87(10sup9/sup/L), and sufficient comparability ( r =?0.73–0.97) with FCM-Ref for each leukocyte differential percentage at 0.4–78.5. The identification ratio of the FCM-Ref in CD45-positive cells was 99.7% (99.4% to 99.8%). Differences were found between FCM-Ref/Celltac G/XN-9000/Spinner-Diff and Wedge-Diff for monocytes and neutrophils. The appearance ratio of smudge cells on wedge and spinner film was 12.5% and 0.5%. Conclusion The Celltac G hematology analyzer's leukocyte differential showed adequate accuracy compared with the CAAs, FCM-Ref, and two manual methods and was considered suitable for clinical use.
机译：背景技术自动血液学分析仪Celltac G（Nihon Kohden）旨在提高白细胞差异性能。与使用不同白细胞检测原理的分析仪的分析仪对楔形膜（楔形件）的分析仪进行比较显示其临床实用性，与免疫型白细胞差分参考方法（FCM-REF）的比较显示了其精度性能。方法比较方法，选择598个临床样品和46个健康志愿者样品。使用的两种比较血液学分析仪（CAAs）是XN-9000（Sysmex）和Cell-Dyn蓝宝石（Abbott）。选择了日本实验室血液学学会提供的FCM-ref，使用流动细胞仪NaviO（Beckman-Coulter）。在手动差动中，使用了两种自动化滑动制造商：用于楔形技术的SP-10（Sysmex），Spinner-2000（Lion-Power）用于旋转工艺技术。旋转器技术通过去除膜上的破碎细胞和血细胞分布的不均匀性（Spinner-Diff）来避免楔形斑块细胞问题。结果Celltac G显示出足够的可比性（R =Δ0.67-1.00），用于每个白细胞差分计数值的CAA，0.00-40.87（10＆ / sup＆ / l），以及足够的可比性（r = 0.73 -0.97）对于每种白细胞差异百分比的FCM-REF，0.4-78.5。 CD45阳性细胞中FCM-REF的鉴定率为99.7％（99.4％至99.8％）。在FCM-REF / Celltac G / XN-9000 / Spinner-Diff和楔形衍生之间发现差异，用于单核细胞和中性粒细胞。楔形细胞对楔和纺纱膜的外观比为12.5％和0.5％。结论与CAAS，FCM-REF和两种手动方法相比，Celltac G血液分析仪的白细胞差异表现出足够的准确度，并认为适用于临床使用。

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