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首页> 外文期刊>Journal of King Saud University >Myrtus communis and its bioactive phytoconstituent, linalool, interferes with Quorum sensing regulated virulence functions and biofilm of uropathogenic bacteria: In vitro and in silico insights
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Myrtus communis and its bioactive phytoconstituent, linalool, interferes with Quorum sensing regulated virulence functions and biofilm of uropathogenic bacteria: In vitro and in silico insights

机译： myrtus communis 及其生物活性植物植物，Linalool，干扰尿致血管毒力功能和生物膜的尿致原细菌：体外，斜体>斜体>和在硅中见解

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Ever increasing spread of drug resistance among bacterial pathogens have rendered the current antibiotic therapy ineffective. Drug-resistant urinary tract infections caused by Gram negative bacterial pathogens such asPseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii,andSerratia marcescensare considered more severe and life threatening because of strong biofilm dependent growth characteristics among majority of them. Moreover, most uropathogens coordinate their complex virulence responses via a highly structured network of cell to cell communication known as Quorum sensing (QS). Phytochemicals/bioactive extracts obtained from established ethnomedicinal plants have previously been demonstrated as effective anti-QS agent against numerous pathogenic bacteria including uropathogens.Myrtus communis(L.) (Myrtaceae) is one such plant of immense ethnomedicinal importance and has been used in traditional medicine. In the present investigation, methanolic extract ofMyrtus communis(MCME) demonstrated 65% inhibition in the QS regulated violacein production inC. violaceumwithout affecting the growth of the bacteria. Further, the MCME interfered significantly with QS controlled virulence production inP. aeruginosa(elastase, protease, pyocyanin and chitinase) andS. marcescens(prodigiosin and protease). The leaf extract exhibited 16–74%, 31–84%, 12–66% and 19–71% inhibition of biofilm biomass ofP. aeruginosa,E. coli,A. baumanniiandS. marcescensrespectively at the increasing concentration corresponding to MIC/16-MIC/2. GC–MS analysis revealed Linalool as one of the major bioactive compounds present in the MCME. Further,in vitroassays demonstrated QS and biofilm inhibition by sub-MICs of linalool.In silicodocking and simulation studies showed that the possible mechanisms responsible for the interference of QS were AHL synthesis inhibition, antagonization of QS-regulatory proteins, and blocking of the receptor proteins. Thus, from the obtained results it is envisaged thatM. communisand its bioactive compound linalool may have medicinal implications and could prove as effective therapeutic agent in combating the threat of biofilm based persistent infections caused by uropathogenic bacteria.
机译：细菌病原体之间的耐药性差异越来越多地使目前的抗生素治疗无效。耐药尿路感染由革兰氏阴性细菌病原体引起的aspseudomonas铜绿假单胞菌，大肠杆菌，肺菌，治疗症，胚乳，并且由于它们中大多数的生物膜依赖性增长特征而被认为更严重和生命。此外，大多数尿肠蛋蛋白通过高度结构化的细胞网络与称为仲裁感测（QS）的细胞通信协调其复杂的毒力响应。从已建立的乙型植物中获得的植物化学物质/生物活性提取物之前已经证明是针对许多致病性细菌的有效抗QS剂，包括尿肠甘液.MYRTUS Communis（L。）（myrtaceae）是巨大的乙烯神奇重要性的一种，并且已用于传统医学。在本研究中，Myrtus Communis（MCME）的甲醇提取物在QS规定的Violach生产公司中展示了65％的抑制作用。 violaceumwithout影响细菌的生长。此外，MCME对QS控制的毒力产生INP显着干扰。铜绿假单胞菌（弹性蛋白酶，蛋白酶，碧糊酶和几丁质酶）。马氏体（Prodigiosin和Protease）。叶提取物显示出16-74％，31-84％，12-66％和19-71％的生物膜生物质的抑制。铜绿假单胞菌，e。大肠杆菌，a。 Baumanniiands。在对应于MIC / 16-MIC / 2的浓度增加的浓度下毫微苛刻。 GC-MS分析显示Linalool作为MCME中存在的主要生物活性化合物之一。此外，在vitroassays中，通过Linalool亚麦克解物证明了QS和生物膜抑制。硅陶器和仿真研究表明，负责QS干扰的可能机制是AHL合成抑制，QS调节蛋白的拮抗，以及阻断受体蛋白。因此，从所获得的结果中设想。 Communisand其生物活性化合物LILALOOL可能具有药物影响，并且可以作为有效的治疗剂，在调用尿致原细菌引起的基于生物膜的持续感染威胁中的有效治疗剂。

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