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Saccharomyces cerevisiae extracts improved the effects of a low fishmeal, complex plant protein diet in the orange-spotted grouper, Epinephelus coioides

机译:酿酒酵母酿酒酵母提取物改善了橙色斑点的石斑鱼,epinephelus coioides中低鱼粉,复杂的植物蛋白饮食的影响

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Six iso-nitrogenous and iso-lipidic experimental diets were formulated to evaluate the effects of Saccharomyces cerevisiae extracts, as part of a low fishmeal diet with a complex plant protein source, on the orange-spotted grouper ( Epinephelus coioides ). In positive control group, fishmeal was the only protein source (FM). The negative control group (Y0) was fed low amounts of fishmeal, and plant protein was used as the protein source. The other diets were supplemented with Saccharomyces cerevisiae extract: 1 g/kg (Y1), 2 g/kg (Y2), 5 g/kg (Y5), and 10 g/kg (Y10). The Epinephelus coioides were divided into six groups, with three replicates per group and 30 groupers per replicate. The experiment lasted for 56 days. The results showed that there was no significant difference in weight gain rate and specific growth rate of FM, Y0 and Y2 groups ( P 0.05). In foregut, mucosal folds length in Y2 group was significantly greater than that in Y0, Y1 and Y10 groups ( P 0.05). The muscle thicknesses of foregut in Y2 and Y5 groups were significantly higher than those in other groups ( P 0.05). The muscle thicknesses of midgut and hindgut in Y2 group were significantly higher than those in Y0 group ( P 0.05). IL-1β and TNF-α in Y1 group were significantly downregulated compared with Y0 group ( P 0.05). After Vibrio harveyi challenge, survival rate in Y1 and Y10 groups was significantly greater than that in Y0 group ( P 0.05). These results indicated that compared with the negative control group, the addition of 2 g/kg S. cerevisiae extract could increase the mucosal folds length of the foregut, and the muscle thickness of the entire intestine, and maintain the growth similar to that of the positive control group. Adding S. cerevisiae extract to feed may improve the immune capacity of E. coioides , but the improvement was not obvious under the challenge of V. harveyi .
机译:配制六种异氮和异脂肪酸实验饮食以评估酿酒酵母提取物的作用,作为橙色斑点蛋白质源(Epinephelus Coioides)的复杂植物蛋白来源的低鱼粉饮食的一部分。在阳性对照组中,鱼粉是唯一的蛋白质来源(FM)。阴性对照组(Y0)送入少量的鱼粉,使用植物蛋白作为蛋白质来源。其他饮食补充有酿酒酵母提取物:1g / kg(Y1),2g / kg(Y2),5g / kg(Y5)和10g / kg(Y10)。将肾上腺素的CoIaides分成6组,每组三种重复,每次重复30个分组。实验持续了56天。结果表明,FM,Y0和Y2组重量增益率和特异性生长速率没有显着差异(P&GT; 0.05)。在前ut中,Y2组中的粘膜折叠长度明显大于Y0,Y1和Y10基团(P <0.05)。在Y2和Y5基团中的前述的肌厚度明显高于其他基团(P <0.05)。 Y2基团中肠和后肠的肌厚度明显高于Y0组(P <0.05)。与Y0基团相比,Y1组中的IL-1β和TNF-α在Y0组比较(P <0.05)中明显下调。在髋菌攻击之后,Y1和Y10组的存活率明显大于Y0组(P <0.05)。这些结果表明,与阴性对照组相比,添加2g / kg酿酒酵母提取物可以增加前述内部的粘膜折叠长度,以及整个肠的肌肉厚度,并保持与之相似的生长阳性对照组。加入酿酒酵母提取物饲料可提高E. CoIaides的免疫能力,但在V.Rvveyi的挑战下,改善并不明显。

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