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Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures

机译：在淡水鱼培养中使用芽孢杆菌淀粉氨酰胺的安全评估

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The primary objective of this work was to assess the effect of Bacillus amyloliquefaciens on the microbiota in polyculture tanks stocked with grass carp, gibel carp and sliver carp. The B. amyloliquefaciens showed high biosafety toward freshwater fish. The addition of B. amyloliquefaciens to the tanks significantly reduced ammonia, total nitrogen, total phosphorus and chemical oxygen demand ( p ＜?0.05) although nitrites increased ( p ＜?0.05). Bacterial diversity in the tanks was greatest in the un-inoculated tanks where Proteobacteria, Actinobacteria, Cyanobacteria, Verrucomicrobia and Bacteroidetes dominated. In the presence of added B. amyloliquefaciens, Proteobacteria and Bacteroidetes levels increased while Actinobacteria decreased. The classes and genera between controls and treatment groups differed although Bacillus levels did not increase in the inoculated tanks. We conclude that supplementation with B. amyloliquefaciens in aquaculture water had many advantages but also potential safety risks.
机译：这项工作的主要目标是评估芽孢杆菌淀粉醇素硫蒜素对用草鲤鱼，吉布斯鲤鱼和锡鲤来储存的多生物坦。 B.淀粉醇提取物向淡水鱼呈现出高生物安全性。虽然亚硝酸盐升高，但向罐中加入罐中的B.淀粉醇提集到罐中显着降低了氨，总氮，总磷和化学需氧量（P <0.05）（P <0.05）。在未接种的坦克中，坦克中的细菌多样性最大，其中植物，肌动菌，肌细胞，疣状细菌，疣状病症和菌丝占主导地位。在添加的B.淀粉氨酸淀粉酮，噬菌体和菌株水平随着肌动菌的增加而增加。虽然芽孢杆菌水平在接种罐中没有增加，但对照组和治疗组之间的类和属性不同。我们得出结论，水产养殖水中的B.淀粉醇素取曲的补充具有许多优点，也具有许多潜在的安全风险。

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