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首页> 外文期刊>Journal of surgery and medicine. >Histological investigation of the protective effect of metformin on testis and sperm parameters in obese rats with type 2 diabetes mellitus
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Histological investigation of the protective effect of metformin on testis and sperm parameters in obese rats with type 2 diabetes mellitus

机译：二甲双胍对2型糖尿病大鼠睾丸和精子参数的组织学研究

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Background/Aim: Diabetes mellitus (DM) is thought to have adverse effects on the male reproductive system. Metformin (MTF) is a widely used drug in the treatment of DM. This study hypothesized that MTF can reduce the harmful effects of DM on the male reproductive system in addition to diabetes treatment. Methods: Twenty-one adult (15-18 g, 11-12 months) C57BL6 male mice were randomly assigned to three groups, as follows: The Control group (C), Diabetes group (D), and Diabetes + Metformin (D+MTF) treatment group. Groups D and D+MTF were fed a diet composed of 60% fat for four weeks. DM was created by administering a single dose of 30 mg/kg streptozotocin intraperitoneally (i.p.). MTF was given through gavage at a dose of 300 mg/kg/day. At the end of the experiment, sperm parameters were evaluated in the testicular tissue. Histomorphology features and immune expressions of VEGF, Caspase-3, and Ki-67 were evaluated in testicular tissue sections stained with Hematoxylin and Eosin (HE). Results: Sperm concentration, motility, and morphological characteristics of diabetic mice were significantly reduced compared to control, and MTF-treated mice. Seminiferous tubule diameters and Johnsen scores were significantly higher in the control and D+MTF groups when compared to the diabetic group. VEGF and Ki-67 immune expression were significantly higher in the control and D+MTF groups compared to the D group (P=0.001, and P=0.002, respectively). Because of D, Caspase-3 positive cell density was higher in the D group compared to the control and D+MTF groups (P=0.002). Conclusion: Diabetes mellitus had adverse effects on sperm parameters and the testicular tissue. High glucose levels with decreased VEGF immune expression damaged spermatogenic cells and decreased proliferation and differentiation due to impaired intratesticular vascularity. MTF treatment reduced the damage in spermatogenic cells and contributed to the differentiation and division of cells through the restoration of vascularization.
机译：背景/目的：糖尿病（DM）被认为对雄性生殖系统产生不利影响。二甲双胍（MTF）是一种广泛使用的药物治疗DM。本研究假设除糖尿病治疗外，MTF还可以降低DM对男性生殖系统的有害影响。方法：二十一体成人（15-18克，11-12个月）C57BL6雄性小鼠随机分配给三组，如下：对照组（C），糖尿病组（D）和糖尿病+二甲双胍（D + MTF）治疗组。将D和D + MTF组喂食为60％脂肪组成的饮食四周。通过腹膜内（I.P）给予单剂量为30mg / kg链脲佐菌素来创建DM。通过饲养剂量为300mg / kg /天的剂量给予MTF。在实验结束时，在睾丸组织中评估精子参数。在用苏木精和曙红（HE）染色的睾丸组织切片中评估了VEGF，Caspase-3和KI-67的组织形态特征和免疫表达。结果：与对照和MTF处理的小鼠相比，糖尿病小鼠的精子浓度，运动和形态特征显着降低。与糖尿病群相比，对照和D + MTF组中，测定的小管直径和约翰顿分数显着高。与D组相比，对照和D + MTF基团的VEGF和KI-67免疫表达显着高于（P = 0.001，分别为0.002）。由于D，与对照和D + MTF基团（P = 0.002）相比，D组中的Caspase-3正细胞密度较高。结论：糖尿病对精子参数和睾丸组织具有不利影响。高葡萄糖水平随着VEGF免疫表达减少受损的精子发生细胞，并且由于内部内部血管受损而降低增殖和分化。 MTF治疗减少了精子发生细胞的损伤，并通过恢复血管化造成细胞的分化和分裂。

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