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首页> 外文期刊>Digestive Diseases and Sciences >Functional Expression of μ-Opioid Receptors in the Human Colon Cancer Cell Line, HT-29, and their Localization in Human Colon
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Functional Expression of μ-Opioid Receptors in the Human Colon Cancer Cell Line, HT-29, and their Localization in Human Colon

机译：μ阿片受体在人结肠癌细胞系HT-29中的功能表达及其在人结肠中的定位

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摘要

We have investigated the functional expression of μ-opioid receptors (MORs) in the human colon cancer cell line, HT-29. As revealed by immunocytochemistry, immunoreactivity was present in both the cytoplasm and nuclei of the cells. Challenge with morphine for 24 h (1 nM to 1 μM) barely affected cell proliferation, while the secretion of urokinase type plasminogen activator (a protease involved in invasion/metastasis) was markedly augmented by a concentration of 0.1 μM. Human colon cancer tissue from 14 consecutively operated patients was investigated by immunohistochemistry. MORs were found in the nuclei of colonocytes and immune cells of the lamina propria in tumor-free tissue. In tumor tissue, immunoreactivity was found in the membrane and often in the nuclei of tumor cells. The current findings suggest that morphine administration could affect tumor progression by interfering with, for example, invasive properties. Our demonstration of a nuclear expression of the MORs appears to be a novel finding.
机译：我们研究了人类结肠癌细胞系HT-29中μ阿片受体（MOR）的功能表达。如免疫细胞化学所揭示，免疫反应性存在于细胞的细胞质和细胞核中。用吗啡攻击24小时（1 nM至1μM）几乎不影响细胞增殖，而浓度为0.1μM明显增加了尿激酶型纤溶酶原激活剂（一种参与入侵/转移的蛋白酶）的分泌。通过免疫组织化学研究了来自14名连续手术患者的人结肠癌组织。在无肿瘤组织的固有层的结肠细胞和免疫细胞的细胞核中发现了MORs。在肿瘤组织中，免疫反应性存在于肿瘤细胞的膜和细胞核中。目前的发现表明吗啡的给药可以通过干扰例如侵袭性来影响肿瘤的进展。我们对MORs核表达的证明似乎是一个新颖的发现。

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