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Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum.

机译:用于定量人分泌物和血清中的免疫球蛋白A的免疫酶联免疫吸附测定。

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We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.
机译:我们开发了一种用于定量人类分泌物和血清中的人免疫球蛋白A的酶联免疫吸附试验。该方法使用免疫形状作为固相,具有10ng / ml的敏感性,并且可以在1天内完成。由于通过琼脂没有扩散,因此测定在测量分泌免疫球蛋白A时的测定不需要校正因子。该方法应该用于研究人类中局部粘膜免疫的研究,特别是当需要非常敏感的测定时。

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