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首页> 外文期刊>Molecular and Cellular Biology >Molecular characterization of novel reciprocal translocation t(6;14) in an Epstein-Barr virus-transformed B cell precursor.
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Molecular characterization of novel reciprocal translocation t(6;14) in an Epstein-Barr virus-transformed B cell precursor.

机译：在Epstein-BART病毒转化的B细胞前体中新型互易易位T（6; 14）的分子表征。

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An in vitro culture of FLEB14 cells, an Epstein-Barr virus-transformed B cell precursor containing the germ line immunoglobulin genes, gave rise to a uniclonally expanded variant, FLEB14 delta 3, which was rearranged at the immunoglobulin heavy-chain gene locus. Cytogenetic analysis showed that FLEB14 delta 3 had a novel reciprocal translocation, t(6;14)(q15;q32). Molecular cloning of the rearranged DNA fragments and determination of their nucleotide sequence revealed that the recombination event was reciprocal, imprecise, and nonhomologous and took place in the S mu region, like those found in Burkitt's lymphoma cells. We propose a molecular model to explain this genetic event which may be relevant to class switch recombination. The translocated sequence of chromosome 6 did not contain any known oncogenes, although the sequence is conserved among mammals. FLEB14 delta 3 did not show tumorigenicity.
机译：FleB14细胞的体外培养物，含有含生殖系免疫球蛋白基因的Epstein-BARR病毒转化的B细胞前体，产生了单瓣膨胀的变体FLEB11Δ3，其在免疫球蛋白重链基因座上重新排列。细胞遗传学分析表明，FleB14Δ3具有新的互易易位，T（6; 14）（Q15; Q32）。重新排列的DNA片段的分子克隆和它们的核苷酸序列的测定表明，重组事件是互核，不精确和非博学，并且在S亩地区发生，如在Burkitt的淋巴瘤细胞中发现的那些。我们提出了一种分子模型来解释该遗传事件，这可能与阶级开关重组相关。染色体6的易分配序列不含任何已知的癌基因，尽管序列在哺乳动物之间保守。 Fleb14 Delta 3没有显示出肿瘤性。

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