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首页> 外文期刊>Journal of bacteriology >Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.
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Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.

机译：大肠杆菌铁调节CIR基因的克隆和启动子鉴定。

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The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene encoding a 32,000-dalton polypeptide was located downstream of and adjacent to cir, but did not appear to be part of the same transcriptional unit. A 525-base-pair fragment from the 5' end of the 1.8-kilobase-pair receptor-coding region directed iron-regulated transcription and translation of a hybrid cir-lacZ gene. Two overlapping promoters were identified by determination of the transcriptional start sites and by sequence analysis. A small open reading frame (120 nucleotides) of unknown significance preceded the receptor-coding sequence. Examination of the amino acid sequence of the receptor purified from the outer membrane revealed that the gene product was processed by removal of a signal peptide and that the mature form had an amino acid sequence near its amino terminus which closely resembled that of several other TonB-dependent proteins.
机译：将CIR基因编码在细胞铁含量和生长温度下，克隆到多拷贝数质粒中。物理映射和互补分析在大肠杆菌染色体上建立了MGL和NFO之间的CIR的位置。编码32,000-dalton多肽的基因位于CIR的下游和相邻的下游，但似乎没有是相同转录单元的一部分。来自1.8千碱基对受体编码区的5'末端的525碱基对片段，其指导铁调节转录和杂交CiR-LacZ基因的翻译。通过测定转录起始位点并通过序列分析来鉴定两个重叠启动子。在受体编码序列之前，一个未知意义的小开口读数框架（120个核苷酸）。从外膜纯化的受体的氨基酸序列显示，通过除去信号肽来处理基因产物，并且成熟形式在其氨基末端附近具有氨基酸序列，这与其他几种相似的氨基酸序列依赖性蛋白质。

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