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首页> 外文期刊>Environmental Pollution >Detoxification of ochratoxin A by Lysobacter sp. CW239 and characteristics of a novel degrading gene carboxypeptidase cp4
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Detoxification of ochratoxin A by Lysobacter sp. CW239 and characteristics of a novel degrading gene carboxypeptidase cp4

机译：溶杆菌属对曲霉毒素A的解毒作用。 CW239和新型降解基因羧肽酶cp4的特性

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摘要

Ochratoxin A (OTA) is a potent mycotoxin that frequently contaminates agro-products and threatens food safety. A highly efficient OTA degrading strain Lysobacter sp. CW239 was isolated, and the OTA degradation characteristics were investigated. A novel OTA degrading gene carboxypeptidase cp4 was successfully cloned and characterized from CW239. The heterologous recombinant was constructed by gene cp4 and expression vector pET-32a((+)) and overexpressed by E. coli BL21 CodonPlus (TM) (DE3). The recombinant protein rCP4 was purified, and the OTA-degrading activity was evaluated. Although OTA was efficiently degraded by CW239 (24-h degradation ratio of 86.2%), the 24-h OTA degradation ratio for rCP4 was only 36.8% at fairly high concentration (0.25 mg/mL) protein. The degraded product was obtained by immune affinity column (IAC) and determined by mass spectrometry (MS), and the degraded product was the less toxic ochratoxin alpha (OT alpha). Based on the serial investigations of this study, OTA might be simultaneously co-degraded by CP4 and another unknown degrading agent in that degrading strain. (C) 2019 Elsevier Ltd. All rights reserved.
机译：ch曲霉毒素A（OTA）是一种有效的霉菌毒素，经常污染农产品并威胁食品安全。高效OTA降解菌株Lysobacter sp。分离CW239，并研究OTA降解特性。从CW239成功克隆并鉴定了一种新型的OTA降解基因羧肽酶cp4。异源重组体由基因cp4和表达载体pET-32a（（+））构建，并由大肠杆菌BL21 CodonPlus（TM）（DE3）过表达。纯化重组蛋白rCP4，并评估OTA降解活性。尽管CW239可有效降解OTA（24小时降解率为86.2％），但在相当高浓度（0.25 mg / mL）的蛋白质下，rCP4的24小时OTA降解率仅为36.8％。降解产物通过免疫亲和柱（IAC）获得，并通过质谱（MS）测定，降解产物为毒性较小的曲霉毒素α（OT alpha）。根据本研究的系​​列研究，CP4和该降解菌株中另一种未知降解剂可能同时降解OTA。 （C）2019 Elsevier Ltd.保留所有权利。

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  • 来源
    《Environmental Pollution》 |2020年第3期|113677.1-113677.10|共10页
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  • 作者单位

    Zhejiang Acad Agr Sci Inst Qual & Stand Agroprod State Key Lab Qual & Safety Agroprod Prepared Hangzhou 310021 Peoples R China;

    Anhui Agr Univ Sch Tea & Food Sci Technol State Key Lab Tea Biol & Utilizat Hefei 230036 Peoples R China;

    Yangtze Univ Coll Anim Sci Jingzhou 434025 Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ochratoxin A; Carboxypeptidase; Gene cloning; Overexpression; Biodegradation;

    机译：ch曲毒素A;羧肽酶;基因克隆;过度表达;生物降解;

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