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首页> 外文期刊>Environmental Pollution >Comments on the ochratoxin A degradation mechanism by Lysobacter sp. CW239 - Wei Wei et al. (2020)
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Comments on the ochratoxin A degradation mechanism by Lysobacter sp. CW239 - Wei Wei et al. (2020)

机译：溶血杆菌对赭曲霉毒素的评论。 CW239 - Wei Wei等。 （2020）

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摘要

This is a research comment on the ochratoxin A (OTA) degradation mechanism by Lysobacter sp. CW239 regarding the previous publication in Environmental Pollution (Wei et al., 2020). Three possible degradation mechanisms were discussed in the referred publication, but without definite evidences, it was not clear which one worked actually. Here, the gene cp4 deficient mutant CW239(Delta cp4) was successfully constructed, and the carboxypeptidase CP4 role on OTA degradation in strain CW239 was validated in vivo. As a result, the mutant CW239(Delta cp4) without gene cp4 showed less than 10% reduction of 24 hrs degradation ratio compared to wide-type strain CW239. After the gene cp4 complemented to CW239(Delta cp4), the complementary strain (+)cp4 recovered the degradation ability to wide-type. The validation result indicated that the third degradation mechanism (i.e., OTA is degraded by joint action of multiple enzymes in CW239) proposed previous (Wei et al., 2020) was correct route for the degradation strain. This commentary was significant to the following studies on the pollutant detoxify strains with similar degradation characters between identified enzyme and the host strain. (C) 2021 Elsevier Ltd. All rights reserved.
机译：这是由溶杆菌属的赭曲霉毒素A（OTA）的降解机理研究评论。关于环境污染先前公布CW239（Wei等，2020）。三种可能的降解机理是在提到的出版物的讨论，但没有确凿证据，目前还不清楚哪一个实际工作。在此，基因CP4缺陷突变CW239（德尔塔CP4）构建成功，并在应变CW239在体内验证上OTA降解羧CP4作用。其结果是，相比于宽型菌株CW239突变CW239（德尔塔CP4）不基因CP4显示的24个小时劣化率减少小于10％。传统的CW239（德尔塔CP4）的CP4基因后，将互补的菌株（+）CP4回收的降解能力宽型。验证结果表明，第三降解机制（即，OTA由CW239多种酶的联合作用而降解）以前提出的（Wei等人，2020年）是为降解应变正确的路由。这篇评论是以下研究显著与鉴定酶和宿主株之间的相似降解字符污染物解毒的菌株。 （c）2021 elestvier有限公司保留所有权利。

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  • 来源
    《Environmental Pollution》 |2021年第7期|117063.1-117063.3|共3页
  • 作者

    Qian Yingying; Zhang Xuanjun; Fei Qingru; Zhou Yu;

  • 作者单位

    Anhui Agr Univ Sch Tea & Food Sci Technol State Key Lab Tea Plant Biol & Utilizat Hefei 230036 Peoples R China;

    Anhui Agr Univ Sch Tea & Food Sci Technol State Key Lab Tea Plant Biol & Utilizat Hefei 230036 Peoples R China;

    Anhui Agr Univ Sch Tea & Food Sci Technol State Key Lab Tea Plant Biol & Utilizat Hefei 230036 Peoples R China;

    Anhui Agr Univ Sch Tea & Food Sci Technol State Key Lab Tea Plant Biol & Utilizat Hefei 230036 Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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