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首页> 外文期刊>Environmental research >The reduction of selenium(Ⅳ) by boreal Pseudomonas sp. strain T5-6-I - Effects on selenium(Ⅳ) uptake in Brassica oleracea
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The reduction of selenium(Ⅳ) by boreal Pseudomonas sp. strain T5-6-I - Effects on selenium(Ⅳ) uptake in Brassica oleracea

机译：北方假单胞菌对硒（Ⅳ）的还原作用

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Selenium (Se) is an essential micronutrient but toxic when taken in excessive amounts. Therefore, understanding the metabolic processes related to selenium uptake and bacteria-plant interactions coupled with selenium metabolism are of high importance. We cultivated Brassica oleracea with the previously isolated heterotrophic aerobic Se(IV)-reducing Pseudomonas sp. T5-6-I strain to better understand the phenomena of bacteria-mediated Se(IV) reduction on selenium availability to the plants. B. oleracea grown on Murashige and Skoog medium (MSsalt agar) with and without of Pseudomonas sp. were amended with Se(IV)/Se-75(IV), and selenium transfer into plants was studied using autoradiography and gamma spectroscopy. XANES was in addition used to study the speciation of selenium in the B. oleracea plants. In addition, the effects of Se(IV) on the protein expression in B. oleracea was studied using HPLC-SEC. TEM and confocal microscopy were used to follow the bacterial/Se-aggregate accumulation in plants and the effects of bacterial inoculation on root-hair growth. In the tests using Se-75 (IV) on average 130% more selenium was translocated to the B. oleracea plants grown with Pseudomonas sp. compared to the plants grown with selenium, but without Pseudomonas sp.. In addition, these bacteria notably increased root hair density. Changes in the protein expression of B. oleracea were observed on the similar to 30-58 kDa regions in the Se(IV) treated samples, probably connected e.g. to the oxidative stress induced by Se(IV) or expression of proteins connected to the Se(IV) metabolism. Based on the XANES measurements, selenium appears to accumulate in B. oleracea mainly in organic C-Se-H and C-Se-C bonds with and without bacteria inoculation. We conclude that the Pseudomonas sp. T5-6-I strain seems to contribute positively to the selenium accumulation in plants, establishing the high potential of Se-0-producing bacteria in the use of phytoremediation and biofortification of selenium.
机译：硒（Se）是必需的微量营养素，但过量摄入时有毒。因此，了解与硒摄取有关的代谢过程以及细菌与植物之间的相互作用以及硒的代谢非常重要。我们用先前分离的减少异养性有氧Se（IV）的假单胞菌sp。种植了芸苔。 T5-6-I菌株可更好地了解细菌介导的硒（IV）还原对植物体内硒利用率的影响。 B. oleracea生长在Murashige和Skoog培养基（MSsalt琼脂）上，有或没有Pseudomonas sp。用Se（IV）/ Se-75（IV）进行了修饰，并使用放射自显影和伽马能谱研究了硒向植物中的转移。此外，还使用XANES研究了油菜芽孢杆菌植物中硒的形态。另外，使用HPLC-SEC研究了Se（IV）对油菜芽胞杆菌中蛋白质表达的影响。用透射电子显微镜和共聚焦显微镜观察植物中细菌/硒聚集体的积累情况以及细菌接种对根毛生长的影响。在使用Se-75（IV）进行的测试中，硒平均平均多转移了130％到假单胞菌（Pseudomonas sp。）与含硒但不含假单胞菌的植物相比，这些细菌明显增加了根毛的密度。在类似于Se（IV）处理的样品中的30-58kDa区域上观察到了油菜芽孢杆菌的蛋白质表达的变化，可能与例如B.S.IV.相关。 Se（IV）诱导的氧化应激或与Se（IV）代谢相关的蛋白质的表达。根据XANES的测量结果，硒似乎会在油菜中积聚，主要存在有机C-Se-H和C-Se-C键，无论是否接种细菌。我们得出结论，假单胞菌属。 T5-6-I菌株似乎对硒在植物中的积累有积极作用，从而在利用硒进行植物修复和生物强化中确立了产生Se-0的细菌的高潜力。

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