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首页> 外文期刊>Environmental research >Assessment of pharmaceutical mixture (ibuprofen, ciprofloxacin and flumequine) effects to the crayfish Procambarus clarkii: A multilevel analysis (biochemical, transcriptional and proteomic approaches)
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Assessment of pharmaceutical mixture (ibuprofen, ciprofloxacin and flumequine) effects to the crayfish Procambarus clarkii: A multilevel analysis (biochemical, transcriptional and proteomic approaches)

机译：评估药物混合物（布洛芬，环丙沙星和FLUMEQUINE）对小龙虾Procambarus clarkii的影响：多级分析（生物化学，转录和蛋白质组学方法）

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摘要

The knowledge about the effects of Pharmaceuticals on aquatic organisms has been increasing in the last decade. However, due to the variety of compounds presents in the aquatic medium, exposure scenarios and exposed organisms, there are still many gaps in the knowledge on how mixtures of such bioactive compounds affect exposed non target organisms. The crayfish Procambarus clarkii was used to analyze the toxicity effects of mixtures of ciprofloxacin, flumequine and ibuprofen at low and high concentrations (10 and 100 μg/L) over 21 days of exposure and to assess the recovery capacity of the organism after a depuration phase following exposure during additional 7 days in clean water. The crayfish accumulated the three compounds throughout the entire exposure in the hepatopancreas. The exposure to the mixture altered the abundance of proteins associated with different cells functions such as biotransformation and detoxification processes (i.e. catalase and glutathione transferase), carbohydrate metabolism and immune responses. Additionally changes in expression of genes encoding antioxidant enzymes and in activity of the corresponding enzymes (i.e. superoxide dismutase, glutathione peroxidase and glutathione transferase) were reported. Alterations at different levels of biological organization did not run in parallel under all circumstances and can be related to changes in the redox status of the target tissue. No differences were observed between control and exposed organisms for most of selected end-points after a week of depuration, indicating that exposure to the drug mixture did not produce permanent damage in the hepatopancreas of P. clarkii.
机译：关于药物对水生生物的影响的了解在过去十年中一直在增加。然而，由于水生培养基中的各种化合物，暴露情景和暴露的生物，仍然存在许多关于这种生物活性化合物的混合物如何影响暴露的非靶毒性的差异的差距。小龙虾procambarus clarkii用于分析在暴露的低温和高浓度（10和100μg/l）上在暴露的低温和高浓度（10和100μg/l）中的毒性作用超过21天，并在剩余阶段评估生物体的恢复能力在清洁水中额外7天内接触。小龙虾在肝癌的整个暴露中积聚了三种化合物。暴露于混合物改变了与不同细胞功能相关的蛋白质的丰度，例如生物转移和解毒方法（即，过氧化氢酶和谷胱甘肽转移酶），碳水化合物代谢和免疫应答。另外，据报道了编码抗氧化酶的基因的表达和相应酶的活性的变化（即超氧化物歧化酶，谷胱甘肽过氧化物酶和谷胱甘肽转移酶）。不同水平的生物组织的改变在所有情况下没有并行运行，并且可以与目标组织的氧化还原状态的变化有关。在剩余一周后，控制和暴露生物之间的控制和暴露生物之间没有差异，表明对药物混合物的暴露不会在P.Clarkii的肝癌中产生永久性损伤。

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  • 来源
    《Environmental research》 |2021年第9期|111396.1-111396.15|共15页
  • 作者

    Chiara Trombini; Julia Kazakova; Alejandro Montilla-Lopez; Ricardo Fernandez-Cisnal; Miriam Hampel; Rut Fernandez-Torres; Miguel Angel Bello-Lopez; Nieves Abril; Julian Blasco;

  • 作者单位

    Department of Ecology and Coastal Management Institute de Ciencias Marinas de Andalucia (CSIC) Campus Rio San Pedro 11510 Puerto Real Cadiz Spain;

    Department of Analytical Chemistry Faculty of Chemistry Universidad de Sevilla 41012 Spain;

    Department of Biochemistry and Molecular Biology Universidad de Cordoba Campus Universitario de Rabanales 14071 Cordoba Spain;

    Department of Biochemistry and Molecular Biology Universidad de Cordoba Campus Universitario de Rabanales 14071 Cordoba Spain;

    Instituto Universitario de Investigation Marina (INMAR) Campus Rio San Pedro 11510 Puerto Real Cadiz Spain;

    Department of Analytical Chemistry Faculty of Chemistry Universidad de Sevilla 41012 Spain;

    Department of Analytical Chemistry Faculty of Chemistry Universidad de Sevilla 41012 Spain;

    Department of Biochemistry and Molecular Biology Universidad de Cordoba Campus Universitario de Rabanales 14071 Cordoba Spain;

    Department of Ecology and Coastal Management Institute de Ciencias Marinas de Andalucia (CSIC) Campus Rio San Pedro 11510 Puerto Real Cadiz Spain;

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  • 正文语种 eng
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  • 关键词

    Procambarus clarkii; Pharmaceuticals mixtures; Biomarkers; Proteomic; Gene expression;

    机译：Procambarus clarkii;药物混合物;生物标志物;蛋白质组学;基因表达;

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