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首页> 外文期刊>Environmental Science & Technology >Detection and Quantification of Photosystem II Inhibitors Using the Freshwater Alga Desmodesmus subspicatus in Combination with High-Performance Thin-Layer Chromatography
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Detection and Quantification of Photosystem II Inhibitors Using the Freshwater Alga Desmodesmus subspicatus in Combination with High-Performance Thin-Layer Chromatography

机译：结合高效薄层色谱法检测和定量使用淡水海藻去皮亚种光子系统的光系统II抑制剂

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摘要

We present a novel tool for detecting and monitoring photosystem II (PSII) inhibitors, using the freshwater alga Desmodesmus subspicatus, in environmental samples fractionated by high-performance thin-layer chromatography (HPTLC). After chromatographic separation of a sample on a HPTLC plate, the algal suspension is sprayed homogeneously on the plate, and PSII-inhibition by specific sample components is detected based on changes in fluorescence yield, viewed by a maxi Imaging-PulseAmplitude-Modulation fluorometer. Dose-dependent responses to the PSII-inhibitor herbicides atrazine and diuron, frequently detected in water bodies, are demonstrated without and with chromatographic separation. The limits of quantification for atrazine and diuron with chromatographic separation were 1.94 ng and 99 pg, respectively, allowing the detection of environmentally relevant concentrations of these herbicides. The developed method was also employed to analyze sample extracts collected during a passive sampling campaign in surface waters. The obtained data correlated well with results from LC-MS/MS chemical analysis but also revealed unknown PSII-inhibiting activities. The proposed methodology represents a rapid and sensitive screening tool for the simultaneous effect-based detection of PSII-inhibitors in environmental samples.
机译：我们提出了一种新型工具，用于通过高效薄层色谱（HPTLC）分离的环境样品中的淡水藻类Desmodesmus subspicatus检测和监测光系统II（PSII）抑制剂。在HPTLC板上色谱分离样品后，将藻类悬浮液均匀喷雾在板上，并通过荧光产量的变化检测特定样品组分对PSII的抑制作用，用maxi Imaging-PulseAmplitude-Modulation荧光计进行观察。在水体中经常检测到的对PSII抑制剂除草剂at去津和杜隆的剂量依赖性反应已得到证实，无需进行色谱分离。通过色谱分离对at去津和杜隆的定量限分别为1.94 ng和99 pg，从而可以检测到这些除草剂的环境相关浓度。所开发的方法还用于分析在被动采样活动中在地表水中收集的样品提取物。所获得的数据与LC-MS / MS化学分析的结果具有很好的相关性，但也揭示了未知的PSII抑制活性。拟议的方法代表了一种快速而灵敏的筛选工具，用于基于效果的同时检测环境样品中的PSII抑制剂。

著录项

  • 来源
    《Environmental Science & Technology》 |2019年第22期|13458-13467|共10页
  • 作者

    Riegraf Carolin; Reifferscheid Georg; Becker Benjamin; Belkin Shimshon; Hollert Henner; Feiler Ute; Buchinger Sebastian;

  • 作者单位

    Fed Inst Hydrol Mainzer Tor 1 D-56068 Koblenz Germany|Rhein Westfal TH Aachen Worringerweg 1 D-52074 Aachen Germany;

    Fed Inst Hydrol Mainzer Tor 1 D-56068 Koblenz Germany;

    Hebrew Univ Jerusalem Inst Life Sci Dept Plant & Environm Sci IL-9190401 Jerusalem Israel;

    Rhein Westfal TH Aachen Worringerweg 1 D-52074 Aachen Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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