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首页> 外文期刊>Environmental Science & Technology >Determining Multiple Responses of Pseudomonas aeruginosa PAO1 to an Antimicrobial Agent, Free Nitrous Acid
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Determining Multiple Responses of Pseudomonas aeruginosa PAO1 to an Antimicrobial Agent, Free Nitrous Acid

机译：测定铜绿假单胞菌PAO1对抗菌剂游离亚硝酸的多重反应

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摘要

Free nitrous acid (FNA) has recently been demonstrated as an antimicrobial agent on a range of microorganisms, especially in wastewater-treatment systems. However, the antimicrobial mechanism of FNA is largely unknown. Here, we report that the antimicrobial effects of FNA are multitargeted The response of a model denitrifier, Pseudomnas aeruginosa PAO1 (PAO1), common in wastewater treatment, was investigated in the absence and presence of inhibitory level of FNA (0.1 mg N/L) under anaerobic denitrifying conditions. This was achieved through coupling gene expression analysis, by RNA sequencing, and with a suite of physiological analyses. Various transcripts exhibited significant changes in abundance in the presence of FNA Respiration was likely inhibited because denitrification activity was severely depleted, and decreased transcript levels of most denitrification genes occurred. As a consequence, the tricarboxylic acid (TCA) cycle was inhibited due to the lowered cellular redox state in the FNA-exposed cultures. Meanwhile, during FNA exposure, PAO1 rerouted its carbon metabolic pathway from the TCA cycle to pyruvate fermentation with acetate as the end product as a possible survival mechanism. Additionally, protein synthesis was significantly decreased, and ribosome preservation was evident These findings improve our understanding of PAO1 in response to FNA and contribute toward the potential application for use of FNA as an antimicrobial agent.
机译：游离亚硝酸（FNA）最近已被证明是对多种微生物的抗微生物剂，尤其是在废水处理系统中。但是，FNA的抗菌机制在很大程度上尚不清楚。在此，我们报道了FNA的抗菌作用是多目标的。在不存在和存在FNA抑制水平（0.1 mg N / L）的情况下，研究了典型的反硝化剂铜绿假单胞菌PAO1（PAO1）在废水处理中的反应。在厌氧反硝化条件下。这是通过偶联基因表达分析，RNA测序以及一系列生理分析来实现的。在存在FNA的情况下，各种转录物显示出丰度的显着变化呼吸作用可能被抑制，因为反硝化活性被严重耗尽，并且大多数反硝化基因的转录物水平降低。结果，由于暴露于FNA的培养物中细胞氧化还原状态降低，三羧酸（TCA）循环受到抑制。同时，在暴露于FNA的过程中，PAO1将其碳代谢途径从TCA循环重新路由到丙酮酸发酵，而乙酸盐作为终产物可能是生存机制。此外，蛋白质合成显着减少，并且核糖体的保存明显。这些发现改善了我们对FAO响应的PAO1的了解，并有助于将FNA用作抗菌剂的潜在应用。

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  • 来源
    《Environmental Science & Technology》 |2016年第10期|5305-5312|共8页
  • 作者

    Shu-Hong Gao; Lu Fan; Lai Peng; Jianhua Guo; Miriam Agullo-Barcelo; Zhiguo Yuan; Philip L. Bond;

  • 作者单位

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia;

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia,iCarbonX, Shenzhen 518053, China;

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia,Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Ghent 9000, Belgium;

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia;

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia;

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia;

    Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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