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首页> 外文期刊>Environmental Science & Technology >Bioaccumulation of Nanosilver by Chlamydomonas reinhardtii- Nanoparticle or the Free Ion?
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Bioaccumulation of Nanosilver by Chlamydomonas reinhardtii- Nanoparticle or the Free Ion?

机译：莱茵衣藻-纳米颗粒或游离离子对纳米银的生物富集？

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摘要

The bioavailability of a small silver nanoparticle (nAg; nominal size of 5 nm with a polyacrylate coating) by the green alga C reinhardtii was investigated in order to assess the contributions of Ag~+ and nAg to cellular internalization. Upon exposure to nAg, Ag biouptake exceeded what was predicted based upon measured Ag~+ concentrations. Indeed, although Ag biouptake was greatly reduced when excess cysteine was added to the nAg, it was nonetheless significantly above control levels. For both exposures to nAg and Ag~+, expression levels of the Copper Transport Protein 2 (CTR2) indicated that Ag biouptake could be attributed to the intemalization of Ag~+. Exposure to Ag~+ or nAg increased CTR2 expression, even when cysteine was present with the nAg. Darkfield microscopy coupled with hyperspectral imagery showed that the presence of silver nanoparticles inside the cells was more likely due to the rereduction of Ag~+ than to the intemalization of nAg. The weight of evidence indicated that nAg increased Ag biouptake by locally increasing the surface concentrations of Ag~+.
机译：研究了绿藻C reinhardtii对小的银纳米颗粒（nAg；标称尺寸为5 nm，聚丙烯酸酯涂层）的生物利用度，以评估Ag〜+和nAg对细胞内在化的贡献。暴露于nAg后，Ag的生物吸收量超过了基于测得的Ag +浓度所预测的水平。确实，尽管将过量的半胱氨酸添加到nAg中后，Ag的生物吸收量大大降低，但仍大大高于对照水平。对于nAg和Ag〜+暴露，铜转运蛋白2（CTR2）的表达水平表明Ag的生物摄取可能归因于Ag〜+的内在化。暴露于Ag〜+或nAg会增加CTR2表达，即使nAg中存在半胱氨酸也是如此。暗场显微镜结合高光谱图像显示，细胞内银纳米颗粒的存在更有可能是由于Ag〜+的还原，而不是nAg的内在化。大量证据表明，nAg通过局部增加Ag〜+的表面浓度来增加Ag的生物吸收。

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  • 来源
    《Environmental Science & Technology》 |2014年第1期|358-364|共7页
  • 作者

    Simon Leclerc; Kevin J. Wilkinson;

  • 作者单位

    Biophysical Environmental Chemistry Group Department of Chemistry, University of Montreal, C.P. 6128 Succursale Centre-ville, Montreal H3C 3J7, Canada;

    Biophysical Environmental Chemistry Group Department of Chemistry, University of Montreal, C.P. 6128 Succursale Centre-ville, Montreal H3C 3J7, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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