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首页> 外文期刊>Environmental Science & Technology >Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches
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Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches

机译：七个大湖海滩的细菌病原体基因丰度及其与游乐水质的关系

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摘要

Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eae_(O157)), shiga-toxin producing E. coli (stxi), Campylobacter jejuni (mapA), Shigella spp. (ipaH), and a Salmonella enterica-specific (SE) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA＞stx2＞ipaH＞SE＞eae_(O157). Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA, stx2, and ipaH detections. Beach seasonal mean map A abundance in water was correlated with beach seasonal mean log_(10) E. coli concentration. At one beach, stxl gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stxl, ipaH, and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management.
机译：大湖区海滩细菌病原体的定量评估，其地理变异性以及在各种基质中的分布是有限的。定量PCR（qPCR）用于测试来自大肠杆菌O157：H7（eae_（O157）），产生志贺毒素的大肠杆菌（stxi），空肠弯曲杆菌（mapA），志贺氏菌属的基因。 （ipaH）和沙门氏菌特异性（SE）DNA序列在七个大湖海滩的藻类，水和沉积物中。总体上，检测频率为mapA＞ stx2＞ ipaH＞ SE＞ eae_（O157）。结果在海滩和矩阵之间变化很大；对于mapA，stx2和ipaH检测，观察到与环境条件的一些相关性。海滩季节性平均值图水中的丰富度与海滩季节性平均值log_（10）大肠杆菌浓度相关。在一个海滩上，stxl基因丰度与同时存在的每日大肠杆菌浓度呈正相关。藻类，沉积物和水中stxl，ipaH和mapA的浓度分布在统计上不同（R中的非检测和数据分析）。假设有10％，50％或100％的基因拷贝代表有活力且可能是感染性细胞，由密歇根州立大学开发的定量微生物风险评估工具表明在研究海滩上空肠弯曲菌的患病可能性中等，尤其是在符合娱乐用水水质标准的地方超出。病原体基因定量可能对海滩水质管理有用。

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  • 来源
    《Environmental Science & Technology》 |2014年第24期|14148-14157|共10页
  • 作者

    Ryan J. Oster; Rasanthi U. Wijesinghe; Sheridan K. Haack; Lisa R. Fogarty; Taaja R. Tucker; Stephen C. Riley;

  • 作者单位

    U.S. Geological Survey, Michigan Water Science Center, Lansing, Michigan 48911, United States;

    U.S. Geological Survey, Michigan Water Science Center, Lansing, Michigan 48911, United States;

    U.S. Geological Survey, Michigan Water Science Center, Lansing, Michigan 48911, United States;

    U.S. Geological Survey, Michigan Water Science Center, Lansing, Michigan 48911, United States;

    CSS-Dynamac, 10301 Democracy Lane, Suite 300, Fairfax, Virginia 22030, United States;

    U.S. Geological Survey, Great Lakes Science Center, Ann Arbor, Michigan 48105, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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