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首页> 外文期刊>Environmental Science & Technology >Surface Cell Density Effects on Escherichia coli Gene Expression during Cell Attachment
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Surface Cell Density Effects on Escherichia coli Gene Expression during Cell Attachment

机译：在细胞附着过程中表面细胞密度对大肠杆菌基因表达的影响

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摘要

Escherichia coli attachment to a surface initiates a complex series of interconnected signaling and regulation pathways that promote biofilm formation and maturation. The present work investigates the effect of deposited cell density on E. coli cell physiology, metabolic activity, and gene expression in the initial stages of biofilm development. Deposited cell density is controlled by exploiting the relationship between ionic strength and bacterial attachment efficiency in a packed bed column. Distinct differences in cell transcriptome are analyzed by comparing sessile cultures at two different cell surface densities and differentiating ionic strength effects by analyzing planktonic cultures in parallel. Our results indicate that operons regulating trypotophan production and the galactitol phosphotransferase system (including dihydroxyacetone phosphate synthesis) are strongly affected by cell density on the surface. Additional transcriptome and metabolomic impacts of cell density on succinate, proline, and pyroglutamic acid systems are also reported. These results are consistent with the hypothesis that surface cell density plays a major role in sessile cell physiology, commencing with the first stage of biofilm formation. These findings improve our understanding of biofilm formation in natural and engineered environmental systems and will contribute to future work ranging from pathogen migration in the environment to control of biofouling on engineered surfaces.
机译：大肠杆菌附着在表面上会引发一系列复杂的相互联系的信号传导和调控途径，从而促进生物膜的形成和成熟。本工作调查生物膜发展的初始阶段沉积的细胞密度对大肠杆菌细胞生理，代谢活性和基因表达的影响。通过利用填充床色谱柱中离子强度和细菌附着效率之间的关系来控制沉积的细胞密度。通过比较两种不同细胞表面密度下的无柄培养物并通过并行分析浮游培养物来区分离子强度效应，从而分析细胞转录组的明显差异。我们的结果表明，调控色氨酸和半乳糖醇磷酸转移酶系统（包括磷酸二羟基丙酮的合成）的操纵子受表面细胞密度的强烈影响。还报道了细胞密度对琥珀酸，脯氨酸和焦谷氨酸系统的其他转录组和代谢组学影响。这些结果与以下假设相符：从生物膜形成的第一阶段开始，表面细胞密度在无柄细胞生理中起主要作用。这些发现提高了我们对自然环境和工程环境系统中生物膜形成的理解，并将为从环境中的病原体迁移到工程表面生物污垢控制等未来工作做出贡献。

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  • 来源
    《Environmental Science & Technology》 |2013年第12期|6223-6230|共8页
  • 作者

    Meagan Mauter; Aaron Fait; Menachem Elimelech; Moshe Herzberg;

  • 作者单位

    Departments of Chemical Engineering and Engineering & Public Policy, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213-3815, United States;

    French Associates Institute for Agriculture & Biotechnology of Drylands, Blaustein Institutes for Desert Research, Ben Gurion University of the Negev, Sede-Boqer Campus, 84990, Israel;

    Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States;

    Department of Desalination and Water Treatment, Zuckerberg Institute for Water Research (ZIWR);

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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