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首页> 外文期刊>Environmental Science & Technology >Identification of Major Dioxin-Like Compounds and Androgen Receptor Antagonist in Acid-Treated Tissue Extracts of High Trophic-Level Animals
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Identification of Major Dioxin-Like Compounds and Androgen Receptor Antagonist in Acid-Treated Tissue Extracts of High Trophic-Level Animals

机译：高营养水平动物经酸处理的组织提取物中主要二恶英样化合物和雄激素受体拮抗剂的鉴定

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摘要

We evaluated the applicability of combining in vitro bioassays with instrument analyses to identify potential endocrine disrupting pollutants in sulfuric acid-treated extracts of liver and/or blubber of high trophic-level animals. Dioxin-like and androgen receptor (AR) antagonistic activities were observed in Baikal seals, common cormorants, raccoon dogs, and finless porpoises by using a panel of rat and human cell-based chemical-activated luciferase gene expression (CALUX) reporter gene bioassays. On the other hand, no activity was detected in estrogen receptor a (ERa)-, glucocorticoid receptor (GR)-, progesterone receptor (PR)-, and peroxisome proliferator-activated receptor γl (PPARγ2)-CALUX assays with the sample amount applied. All individual samples (n - 66) showed dioxin-like activity, with values ranging from 21 to 5500 pg CALUX-2,3,7,8-tetrachIorodibenzo-p-dioxin equivalent (TEQ)/g-lipid. Because dioxins are expected to be strong contributors to CALUX-TEQs, the median theoretical contribution of dioxins calculated from the result of chemical analysis to the experimental CALUX-TEQs was estimated to explain up to 130% for all the tested samples (« = 54). Baikal seal extracts (n = 31), but not other extracts, induced AR antagonistic activities that were 8-150/(g CALUX-flutamide equivalent (FluEQ)/g-Iipid.p,p'-DDE was identified as an important causative compound for the activity, and its median theoretical contribution to the experimental CALUX-FluEQs was 59% for the tested Baikal seal tissues (n = 25). Our results demonstrate that combining in vitro CALUX assays with instrument analysis is useful for identifying persistent organic pollutant-like compounds in the tissue of wild animals on the basis of in vttro endocrine disruption toxicity.
机译：我们评估了体外生物测定与仪器分析相结合的适用性，以鉴定硫酸处理的高营养水平动物肝脏和/或脂肪的提取物中潜在的内分泌干扰污染物。通过使用一组基于大鼠和人类细胞的化学激活的荧光素酶基因表达（CALUX）报告基因生物测定法，在贝加尔湖海豹，普通cor，狗和无尾海豚中观察到了二恶英样雄激素受体（AR）的拮抗活性。另一方面，在应用了样品量的雌激素受体a（ERa），糖皮质激素受体（GR），孕激素受体（PR）和过氧化物酶体增殖物激活受体γl（PPARγ2）-CALUX分析中未检测到活性。 。所有样品（n-66）均显示出二恶英样活性，其值范围为21到5500 pg CALUX-2,3,7,8-四氯二苯并-对二恶英当量（TEQ）/ g-脂质。由于预计二恶英是CALUX-TEQ的主要贡献者，因此，根据化学分析结果计算出的二恶英的理论中值对实验CALUX-TEQ的贡献，据估计可解释所有测试样品的最高130％（«= 54） 。贝加尔湖海豹提取物（n = 31），但没有其他提取物诱导的AR拮抗活性为8-150 /（g CALUX-氟他胺当量（FluEQ）/g-Iipid.p,p'-DDE，被认为是重要的病因化合物的活性，对于测试的贝加尔湖海豹组织，其对实验CALUX-FluEQs的中位理论贡献为59％（n = 25）我们的结果表明，将体外CALUX分析与仪器分析相结合可用于鉴定持久性有机污染物类动物体内外分泌破坏毒性基础上的类化合物。

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  • 来源
    《Environmental Science & Technology》 |2011年第23期|p.10203-10211|共9页
  • 作者

    Go Suzuki; Nguyen M. Tue; Sander van der Linden; Abraham Brouwer; Bart van der Burg; Martin van Velzen; Marja Lamoree; Masayuki Someya; Shin Takahashi; Tomohiko Isobe; Yuko Tajima; Tadasu K. Yamada; Hidetaka Takigami; Shinsuke Tanabe;

  • 作者单位

    Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies, Tsukuba, Japan,Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    BioDetection Systems b.v., 1098 XH Amsterdam, The Netherlands;

    BioDetection Systems b.v., 1098 XH Amsterdam, The Netherlands,Faculty of Earth and Life Sciences, VU University, 1081 HV Amsterdam, The Netherlands;

    BioDetection Systems b.v., 1098 XH Amsterdam, The Netherlands;

    Institute for Environmental Studies, VU University, 1081 HV Amsterdam, The Netherlands;

    Institute for Environmental Studies, VU University, 1081 HV Amsterdam, The Netherlands;

    Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    National Museum of Nature and Science, Tokyo 110-8718, Japan;

    National Museum of Nature and Science, Tokyo 110-8718, Japan;

    Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies, Tsukuba, Japan;

    Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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