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Evaluation of Chemical, Molecular, and Traditional Markers of Fecal Contamination in an Effluent Dominated Urban Stream

机译:污水为主的城市溪流中粪便污染的化学,分子和传统标记的评估

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摘要

In this paper we present a quantitative sanitary survey of the Middle Santa Ana River, in southern California, utilizing a variety of source tracking tools, including traditional culture-dependent fecal markers {Enterococcus and Escherichia coli by IDEXX), speciation of enterococci isolates, culture-independent fecal markers (human-specific HF183 Bacteroides ami Enterococcus by quantitative polymerase chain reaction, qPCRI, and chemical markers of sewage and wastewater (nutrients, enantiomeric fraction (EF) of propranolol and ethylenediaminetetraacetic acid). To facilitate comparison of these different methods, data are interpreted in a loading (i.e., mass per time) framework that enables a quantitative apportionment of fecal markers and nutrients to specific source waters in the Middle Santa Ana River. Multiple lines of evidence support the hypothesis that Enterococcus in the Middle Santa Ana River originates primarily from in situ growth in streambed sediments, not from significant and persistent sources of untreated human waste. The EF of propranolol of tertiary treated wastewater effluent is in the range (0.42 to 0.71) previously reported for raw sewage, making EF of propranolol an unsuitable marker for fecal pollution, at least at this site. The human fecal marker HF183 Bacteroides was detected at a few sites, although not in a source of disinfected and tertiary treated wastewater effluent Based on the results presented here and prior experience at other sites in southern California, HF183 Bacteroides would appear to be a candidate marker of fecal contamination for inland waters, although more qPCR measurements in disinfected wastewater effluent are needed to account for variations due to treatment plant performance and other factors. More generally, our results support the notion that regrowth of fecal indicator bacteria (FIB) in river sediments may lead to a decoupling between FIB and pathogen concentrations in the water column and thus limit the utility of FIB as an indicator of recreational waterborne illness in inland waters.
机译:在本文中,我们介绍了加利福尼亚南部中部圣安娜河的定量卫生调查,该调查使用了多种来源跟踪工具,包括传统的依赖于文化的粪便标记物(IDEXX的肠球菌和大肠杆菌),肠球菌分离物的形态,文化-独立的粪便标记物(通过定量聚合酶链反应,qPCRI鉴定的人类特异性HF183细菌肠球菌,以及污水和废水中的化学标记物(营养成分,普萘洛尔和乙二胺四乙酸的对映体分数(EF))。为便于比较这些不同方法,数据在一个加载(即每时间质量)框架中进行解释,从而可以将粪便标志物和营养物定量分配到圣塔安娜河中部的特定水源。多行证据支持这一假设,即圣塔安娜河中部的肠球菌主要来源于河床沉积物的原位生长,而不是大量的以及未经处理的人类废物的持续来源。经三级处理的废水的普萘洛尔的EF在先前报道的原污水范围内(0.42至0.71),这使得普萘洛尔的EF至少在此地点不适合用于粪便污染。在一些地点检测到了人类粪便标记物HF183杀菌剂,尽管未在经过消毒和三级处理的废水中发现。根据此处提供的结果以及在加利福尼亚南部其他地点的先前经验,HF183杀菌剂似乎是候选标记物尽管内陆水域的粪便污染程度较高,但仍需要对消毒废水进行更多的qPCR测量,以说明由于处理厂性能和其他因素而引起的变化。更普遍地说,我们的结果支持以下观点:粪便指示剂细菌(FIB)在河流沉积物中的再生可能导致FIB与水柱中病原体浓度之间脱钩,从而限制了FIB作为内陆娱乐性水生疾病指标的效用水域。

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  • 来源
    《Environmental Science & Technology》 |2010年第19期|p.7369-7375|共7页
  • 作者单位

    Interdisciplinary Program in Environmental Engineering,Department of Chemical Engineering and Materials Science,University of California, Irvine, California 92697;

    rnInterdisciplinary Program in Environmental Engineering,Department of Chemical Engineering and Materials Science,University of California, Irvine, California 92697;

    rnInstitute for Computational Earth System Science, University of California, Santa Barbara, California 93106;

    rnInstitute for Computational Earth System Science, University of California, Santa Barbara, California 93106 Donald Bren School of Environmental Science & Management, University of California,Santa Barbara, California 93106-5131;

    rnDepartment of Civil and Environmental Engineering,University of California, Berkeley, California 94720;

    rnInterdisciplinary Program in Environmental Engineering,Department of Chemical Engineering and Materials Science,University of California, Irvine, California 92697;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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