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首页> 外文期刊>Environmental Science & Technology >Is Upid Peroxidation Induced By The Aqueous Suspension Of Fullerene C_(60)nanoparticles In The Brains Of Cyprinus Carpio?
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Is Upid Peroxidation Induced By The Aqueous Suspension Of Fullerene C_(60)nanoparticles In The Brains Of Cyprinus Carpio?

机译：鲤鱼脑中富勒烯C_（60）纳米颗粒的水悬浮是否诱导了过氧化氢过氧化作用？

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摘要

This study aimed to clarify whether fullerene C_(60) nanoparticles induced lipid peroxidation in Cyprinus carpio brains. A stable well-characterized aqueous suspension of C_(60) nanoparticle (diameter: 50th and 95th percentiles, 36 and 95 nm respectively) with 0.1% Tween80 solution was prepared by bead milling. Lipid hydroperoxides (LPO) were measured in vitro in homogenized fish brain tissues containing 33 μg/mg-protein dispersed C_(60) nanoparticles under light and dark conditions to verify the lipid peroxidation ability of C_(60) and the interference of light exposure by using a commercial assay kit. The LPO concentration significantly increased under the light condition but not under the dark condition. This suggests that C_(60) has the lipid peroxidation ability under light condition, and the light exposure that occurs during the dissection and preparation of fish brain samples containing C_(60) for the LPO assay interferes with the measurements of the in vivo LPO levels. Therefore, dissection and assay in the in vivo experiment were conducted under a yellow lamp or dark condition to avoid the interference of light. Moreover, the result of the in vitro test suggests that the LPO assay with irradiation might be a good method for detecting C_(60) in brain tissues. In the in vivo experiment, C. carpio was exposed to 4.5 mg/L nano C_(60) suspension for 48 h, following which the brain LPO concentration was measured. In the in vivo experiment, no fish died or exhibited abnormal symptoms during exposure. LPO assay of the C. carpio brain samples confirmed the absence of lipid peroxidation after exposure to 4.5 mg/L aqueous C_(60) nanoparticle suspension for 48 h. Additional LPO assay under irradiation showed that C_(60) did not reach the brain.
机译：这项研究旨在阐明富勒烯C_（60）纳米颗粒是否在鲤鱼大脑中诱导脂质过氧化。通过珠磨法制备了稳定的，特性良好的C_（60）纳米颗粒（直径：分别为第50和95个百分位数，分别为36和95 nm）与0.1％Tween80溶液的水悬浮液。在明亮和黑暗的条件下，在含有33μg/ mg蛋白分散的C_（60）纳米颗粒的均质化鱼脑组织中，在体外测量了脂质过氧化氢（LPO），以验证C_（60）的脂质过氧化能力和光照射对人体的干扰。使用商业化验试剂盒。 LPO浓度在明亮条件下显着增加，而在黑暗条件下则没有。这表明C_（60）在光照条件下具有脂质过氧化能力，并且在剖析和制备包含C_（60）的鱼脑样本以进行LPO测定的过程中发生的光照干扰了体内LPO水平的测量。因此，在体内实验中的解剖和测定是在黄灯或黑暗条件下进行的，以避免光的干扰。此外，体外测试的结果表明，用放射线进行LPO测定可能是检测脑组织中C_（60）的好方法。在体内实验中，鲤鱼暴露于4.5 mg / L纳米C_（60）悬浮液中48 h，然后测量脑LPO浓度。在体内实验中，在接触过程中没有鱼类死亡或表现出异常症状。鲤鱼脑样本的LPO分析证实，暴露于4.5 mg / L的C_（60）纳米水悬浮液48小时后，脂质过氧化作用不存在。放射线下的其他LPO分析表明C_（60）没有到达大脑。

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