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首页> 外文期刊>Environmental Science & Technology >Escherichia Coli 0157:h7 Requires Colonizing Partner To Adhere And Persist In A Capillary Flow Cell
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Escherichia Coli 0157:h7 Requires Colonizing Partner To Adhere And Persist In A Capillary Flow Cell

机译：大肠杆菌0157：h7需要殖民伙伴在毛细血管流动池中粘附和坚持

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摘要

The ability of a strain of waterborne Escherichia coli 0157: H7 to colonize a glass flow cell and develop microcolonies when grown alone and with Pseudomonas aeruginosa PA01 was examined. When introduced alone, planktonic E. coli were unable to attach to the glass surface. When introduced simultaneously with P. aeruginosa (co-inoculation), the two species coadhered to the surface. When E. coli were introduced into a flow cell precolonized with a P. aeruginosa biofilm (precolonized), 10-fold more cells were retained than in the co-inoculated case. Both species were monitored nondestructively by time-lapse confocal microscopy, direct microscopy of the filtered effluent, and effluent plate counts. While more E. coli inrtially adhered in the precolonized system, E. coli microcolony formation occurred only in the co-inoculated system, where E. coli comprised 1% of the total surface-associated biovolume but greater than 50% of the biovolume near the edges of the flow cell. The hydrodynamics in the flow cell were evaluated using the finite volume analysis program CFX, revealing that shear stress was likely important in both initial attachment and steady-state colonization patterns. This research elucidates key factors which promote retention and subsequent biofilm development of E. coli 0157:H7.
机译：检验了水性大肠杆菌0157：H7菌株在单独和铜绿假单胞菌PA01一起生长时在玻璃流动池中定殖并形成微菌落的能力。当单独引入时，浮游大肠杆菌无法附着在玻璃表面上。当与铜绿假单胞菌同时引入（共同接种）时，这两个物种共粘附于表面。当将大肠杆菌引入用铜绿假单胞菌生物膜预先定殖（预先定殖）的流通池中时，保留的细胞比共同接种的情况多10倍。通过延时共聚焦显微镜，过滤后的流出物的直接显微镜和流出板数对这两种物种进行了无损监测。尽管有更多的大肠杆菌在肠道内预先附着在菌落中，但仅在共接种系统中才发生大肠杆菌小菌落的形成，在大肠杆菌中，大肠杆菌占总表面相关生物量的1％，但在菌落附近的生物量大于50％。流通池的边缘。使用有限体积分析程序CFX对流动池中的流体动力学进行了评估，结果表明，剪切应力在初始附着和稳态定植模式中都可能很重要。这项研究阐明了促进大肠杆菌0157：H7保留和随后生物膜形成的关键因素。

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