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首页> 外文期刊>Environmental Science & Technology >Evaluation of the Physiology of Medaka as a Model Animal for Standardized Toxicity Tests of Chemicals by Using mRNA Expression Profiling
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Evaluation of the Physiology of Medaka as a Model Animal for Standardized Toxicity Tests of Chemicals by Using mRNA Expression Profiling

机译：通过使用mRNA表达谱法评估Medaka作为标准动物化学毒性试验模型动物的生理状况

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摘要

The fish acute toxicity test in the Organisation for Economic Co-Operation and Development "OECD Guidelines for Testing of Chemicals" is an essential test for environmental toxicity. Here, we have tried to evaluate the physiology of medaka during these test procedures by using genomics. Genomics technology can provide genome-wide expression profiles of mRNA, and these profiles correspond to the physiology of organisms. Thus, a comparison of mRNA expression profiles gives information on the reproducibility of experimental conditions. Expression profiles of mRNA were measured for medakas maintained within the allowable range of the test conditions and also under extreme conditions beyond the guideline limits. We confirmed the high physiological reproducibility of medaka kept in the recommended conditions of the fish acute toxicity test in the "OECD Guidelines for Testing of Chemicals" from the expression profiles obtained under all experimental conditions, except for the types of feeding.
机译：经济合作与发展组织《经合组织化学品试验准则》中的鱼类急性毒性试验是对环境毒性的必要试验。在这里，我们试图通过基因组学方法在这些测试过程中评估evaluate高的生理。基因组学技术可以提供mRNA的全基因组表达谱，这些谱图与生物体的生理学相对应。因此，mRNA表达谱的比较给出了关于实验条件可重复性的信息。测量了维持在测试条件的允许范围内以及在超出指南极限的极端条件下的花aka的mRNA表达谱。我们根据在所有实验条件下获得的表达谱（饲料类型除外），在“经合组织化学物质测试准则”的鱼类急性毒性试验的推荐条件下，保持了高aka的高生理再现性。

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  • 来源
    《Environmental Science & Technology》 |2009年第10期|3913-3918|共6页
  • 作者

    HITOSHI IWAHASHI; KATSUYUKI KISHI; EMIKO KITAGAWA; KATSUNORI SUZUKI; YASUYUKI HAYASHI;

  • 作者单位

    Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba West, Onogawa 16-1, Tsukuba, Ibaraki 305-8569, Japan;

    Health Technology Research Center,Japan Pulp & Paper Research Institute, Inc., Tokodai 5-13-11,Tsukuba, Ibaraki 300-2635, Japan;

    Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba West, Onogawa 16-1, Tsukuba, Ibaraki 305-8569, Japan;

    Health Technology Research Center,GeneFrontier Corporation, Nihonbashi Kayabacho 3-2-10, Chuo-ku, Tokyo 103-0025, Japan;

    Health Technology Research Center,GeneFrontier Corporation, Nihonbashi Kayabacho 3-2-10, Chuo-ku, Tokyo 103-0025, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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