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首页> 外文期刊>Environmental Science & Technology >Prion Protein Adsorption to Soil in a Competitive Matrix Is Slow and Reduced
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Prion Protein Adsorption to Soil in a Competitive Matrix Is Slow and Reduced

机译：on毒蛋白在竞争性基质中对土壤的吸附缓慢且减少

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摘要

It is likely that the soil environment serves as a stable reservoir of infectious CWD and scrapie prions as well as a potential reservoir of BSE. Prion adsorption to soil could play an important role in prion mobility, proteolysis, and infectivity. We modified previously published methods to quantify adsorbed prions via direct detection and studied prion adsorption to soil and soil minerals as a function of time through 60 days. Prion-infected brain homogenate was used as a complex, relevant prion source. We determined that maximum PrP adsorption requires days or weeks, depending on the soil or mineral, and is 2-5 orders of magnitude lower than previous studies using purified PrP~(Sc) or recPrP. Because PrP adsorption to soil is slow and reduced in tissue homogenate, the possibility of prion transport in soil environments cannot be excluded and requires further investigation. Our results indicate that binding to soil may protect prions from degradation, consistent with prions' longevity in the environment. Adsorption of PrP to sterilized soil did not differ significantly from adsorption to unsterilized soil, which suggests that active biological processes do not significantly affect prion adsorption or degradation in the soil environment.
机译：土壤环境可能是传染性CWD和scrap病毒的稳定储库，也可能是BSE的潜在储库。 on病毒对土壤的吸附可能在病毒的迁移，蛋白水解和感染性中起重要作用。我们修改了以前发表的方法以通过直接检测来量化吸附的ions病毒，并研究了60天后病毒对土壤和土壤矿物的吸附随时间的变化。 on病毒感染的脑匀浆被用作复杂的相关病毒来源。我们确定最大的PrP吸附需要几天或几周，具体取决于土壤或矿物，并且比以前使用纯化的PrP〜（Sc）或recPrP的研究低2-5个数量级。由于PrP吸附到土壤的速度很慢并且组织匀浆减少，因此不能排除of病毒在土壤环境中运输的可能性，需要进一步研究。我们的结果表明与土壤的结合可以保护病毒免于降解，这与病毒在环境中的寿命一致。 PrP对灭菌土壤的吸附与未灭菌土壤的吸附无明显差异，这表明活跃的生物过程不会显着影响病毒在土壤环境中的吸附或降解。

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  • 来源
    《Environmental Science & Technology》 |2009年第20期|7728-7733|共6页
  • 作者

    SAMUEL E. SAUNDERS; JASON C. BARTZ; SHANNON L. BARTELT - HUNT;

  • 作者单位

    Department of Civil Engineering, University of Nebraska-Lincoln, Peter Kiewit Institute, Omaha, Nebraska;

    Department of Medical Microbiology and Immunology, Creighton University, Omaha, Nebraska;

    Department of Civil Engineering, University of Nebraska-Lincoln, Peter Kiewit Institute, Omaha, Nebraska;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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