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首页> 外文期刊>Environmental Science & Technology >Characterization of Methyl Mercury in Dental Wastewater and Correlation with Sulfate-Reducing Bacterial DNA
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Characterization of Methyl Mercury in Dental Wastewater and Correlation with Sulfate-Reducing Bacterial DNA

机译：牙科废水中甲基汞的表征及其与硫酸盐还原细菌DNA的关系

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Dental wastewater (DWW) was collected over two months from a 12-chair clinic and a single-chair office to identify conditions that may affect Hg methylation. DWW was settled for 24 h and samples were collected from the top and bottom of the supernatant to simulate a range of particles that may escape inline traps. Total Hg spanned 5 orders of magnitude (0.02-5000 μM), following a log-normal distribution with p10, p50, and p90 concentration values of 0.24,31 and 4000 μM, respectively; typically well in excess of free aqueous Hg solubility. Methyl Hg was present in high levels (2-270 nM), also following a log-normal distribution with p10, p50, and p90 concentration values of 2.8,17, and 100 nM, respectively. There were no statistically significant differences (90% Cl) in p50 methyl Hg ortotal Hg between the clinic and office. Methyl Hg was predicted from total Hg data by (±95% Cl): Log(Me-Hg) = 0.33(±0.06) × Log(T-Hg) - 2.27(±0.13). Total methyl Hg from DWW to U.S. wastewater collection systems is estimated to be 2-5 kg yr~(-1). Equilibrium speciation modeling predicted that DWW Hg was primarily in sulfide-Hg complexes, except at high total Hg levels where organo-Hg complexes become significant. DNA extracts amplified by quantitative polymerase chain reaction with primers for total eubacteria and sulfate-reducing bacteria (SRB) indicated that the total eubacterial DNA was composed primarily of SRB, and highly significant correlations were found between methyl Hg and both amplified Desulfobacteraceae (p < 0.0001) and Desulfovibrionacaea DNA (p < 0.00001). Both are known Hg methylators. In marked contrast, there was no significant correlation between methyl Hg and amplified Desulfobulbus DNA, a genus generally not known to methylate Hg at high rates. These results strongly suggest that SRB are implicated in DWW Hg methylation.
机译：在两个多月的时间里，从一个有12个座位的诊所和一个有单个座位的办公室收集了牙科废水（DWW），以确定可能影响汞甲基化的条件。使DWW沉降24小时，并从上清液的顶部和底部收集样品，以模拟可能逃脱在线阱的一系列颗粒。总汞跨度为5个数量级（0.02-5000μM），p10，p50和p90浓度值分别为对数正态分布，分别为0.24、31和4000μM。通常远远超过游离的Hg水溶液溶解度。甲基汞的含量很高（2-270 nM），p10，p50和p90浓度值分别为2.8、17和100 nM的对数正态分布。在诊所和办公室之间，p50甲基汞或总汞中无统计学差异（90％Cl）。从总汞数据通过（±95％Cl）预测甲基汞：Log（Me-Hg）= 0.33（±0.06）×Log（T-Hg）-2.27（±0.13）。从DWW到美国废水收集系统的总甲基汞估计为2-5 kg yr〜（-1）。平衡形态模型预测，DWW Hg主要存在于硫化物-Hg络合物中，但在总Hg含量高的地方，有机-Hg络合物变得很重要。通过定量聚合酶链反应与总真细菌和硫酸盐还原菌（SRB）引物定量扩增的DNA提取物表明，总真细菌DNA主要由SRB组成，并且甲基汞与两个扩增的脱硫细菌之间都存在高度显着的相关性（p <0.0001 ）和Desulfovibrionacaea DNA（p <0.00001）。两者都是已知的汞甲基化剂。与之形成鲜明对比的是，甲基汞与扩增的脱硫球DNA之间没有显着相关性，脱硫球DNA是一种通常不知道以高速率甲基化汞的属。这些结果强烈暗示SRB与DWW Hg甲基化有关。

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