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首页> 外文期刊>Environmental Science & Technology >Application of Site-Specific Calibration Data Using the CALUX by XDS Bioassay for Dioxin-like Chemicals in Soil and Sediment Samples
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Application of Site-Specific Calibration Data Using the CALUX by XDS Bioassay for Dioxin-like Chemicals in Soil and Sediment Samples

机译：利用XDS生物测定法通过CALUX在特定地点校准数据在土壤和沉积物样品中的类二恶英类化学物质中的应用

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摘要

The Chemically Activated Luciferase Gene Expression (CALUX) by Xenobiotic Detection Systems (XDS) bioassay was evaluated for the determination of the presence of dioxin and dioxin-like compounds in soil and sediment in two studies conducted under the U.S. Environmental Protection Agency's Superfund Innovative Technology Evaluation Monitoring and Measurement Technologies Program. In the first study, the results were compared with those generated by established laboratory methods (EPA Method 1613B) using high-resolution mass spectrometry (HRMS). The study results demonstrated that the technology could be used to screen for dioxin concentrations above and below threshold values (e.g., less than or greater than 1 or 50 picograms of toxicity equivalents per gram [pg TEQ/g]); however, the results were not linearly correlated to the HRMS results. A second study was initiated to evaluate performance on a site-specific basis. During the second study, the data from the XDS technology were evaluated in four ways: (1) uncalibrated to HRMS, (2) calibrated using an overall statistical model, (3) calibrated using statistical models generated on a site-specific basis, and (4) calibrated using site-specific calibration factors. The results showed that TEQ data produced by the XDS technology were more precise than the data reported during the first study. The second study also demonstrated that site-specific statistical models were better tools for understanding the relationship between the XDS and HRMS data than a single overall model generated from data from multiple sites. Ultimately, site-specific calibration was shown to be the best approach because it was a simple and accurate way of correcting the XDS data and improving comparability with HRMS.
机译：在美国环境保护署超级基金创新技术评估下进行的两项研究中，通过异种生物检测系统（XDS）生物测定法对化学活化的萤光素酶基因表达（CALUX）进行了评估，以确定土壤和沉积物中二恶英和类二恶英化合物的存在。监测和测量技术计划。在第一项研究中，将结果与使用高分辨率质谱（HRMS）的已建立实验室方法（EPA方法1613B）产生的结果进行了比较。研究结果表明，该技术可用于筛选阈值以上和以下的二恶英浓度（例如，每克毒性当量小于或大于1或50皮克[pg TEQ / g]）；但是，结果与HRMS结果不是线性相关的。已启动第二项研究，以根据特定地点评估性能。在第二项研究中，以四种方式评估了来自XDS技术的数据：（1）未校准为HRMS，（2）使用整体统计模型进行校准，（3）使用基于特定地点生成的统计模型进行校准，以及（4）使用特定于现场的校准因子进行校准。结果表明，由XDS技术生成的TEQ数据比第一项研究报告的数据更为精确。第二项研究还表明，特定站点的统计模型比从多个站点的数据生成的单个整体模型是更好的工具，用于了解XDS和HRMS数据之间的关系。最终，特定于现场的校准被证明是最好的方法，因为它是校正XDS数据并提高与HRMS可比性的简单而准确的方法。

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  • 来源
    《Environmental Science & Technology》 |2007年第24期|p.8376-8382|共7页
  • 作者

    AMY DINDAL; ELIZABETH THOMPSON; LAURA AUME; STEPHEN BILLETS;

  • 作者单位

    Battelle, 505 King Avenue, Columbus, Ohio 43201, and U.S. Environmental Protection Agency/National Exposure Research Laboratory, 944 East Harmon Avenue, Las Vegas, Neveda 89119;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
  • 关键词

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