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首页> 外文期刊>Environmental Science & Technology >Validation and Interpretation of CALUX as a Tool for the Estimation of Dioxin-Like Activity in Marine Biological Matrixes
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Validation and Interpretation of CALUX as a Tool for the Estimation of Dioxin-Like Activity in Marine Biological Matrixes

机译：验证和解释CALUX作为估算海洋生物基质中二恶英类活性的工具

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摘要

Among the different analytical tools proposed as an alternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses of polychlorodibenzo-p-dioxin and polychlorodibenzofurans, Chemically Activated LUciferase gene expression (CALUX) in vitro cell bioassay is very promising. It allows the analyses of a high number of samples since it is relatively fast, inexpensive, and sensitive. However, this technique is not yet widely applied for screening or environmental monitoring. The main reasons are probably the lack of validation and the difficulty in interpreting the global biological response of the bioassay. In this paper, the strict quality control criteria set up for the validation of CALUX are described. The validation has shown good repeatability (relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. The quantification limit, in the conditions applied in this paper, is 1.25 pg CALUX-TEQ/g fat. Comparison of CALUX and GC-HRMS analysis was made for various marine matrixes (fishes, mussels, starfishes, sea birds, and marine mammals). Good correlations are usually observed, but there are systematic differences between the results. Attempts are made to identify the origin of the discrepancy between the two methods.
机译：建议使用多种分析工具代替非常昂贵的气相色谱高分辨率质谱（GC-HRMS）分析聚氯二苯并-对-二恶英和聚氯二苯并呋喃，其中化学激活的荧光素酶基因表达（CALUX）体外细胞生物测定非常有前途。由于它相对快速，便宜且灵敏，因此可以分析大量样品。但是，该技术尚未广泛应用于筛选或环境监测。主要原因可能是缺乏验证以及难以解释生物测定的整体生物学反应。在本文中，描述了为验证CALUX而建立的严格质量控制标准。验证显示结果具有良好的重复性（相对标准偏差（RSD）= 9％）和实验室内良好的可重复性（RSD = 15％）。在本文应用的条件下，定量限为1.25 pg CALUX-TEQ / g脂肪。针对各种海洋基质（鱼类，贻贝，海星，海鸟和海洋哺乳动物）进行了CALUX和GC-HRMS分析的比较。通常观察到良好的相关性，但结果之间存在系统差异。试图确定两种方法之间差异的根源。

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