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首页> 外文期刊>Environmental toxicology and chemistry >PROTECTIVE ROLE OF DIETARY N-ACETYLCYSTEINE ON THE OXIDATIVE STRESS INDUCED BY CYLINDROSPERMOPSIN IN TILAPIA (OREOCHROMIS NILOTICUS)
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PROTECTIVE ROLE OF DIETARY N-ACETYLCYSTEINE ON THE OXIDATIVE STRESS INDUCED BY CYLINDROSPERMOPSIN IN TILAPIA (OREOCHROMIS NILOTICUS)

机译：膳食N-乙酰半胱氨酸对环十二烷基蛋白酶诱导的罗非鱼氧化应激的保护作用。

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摘要

Cylindrospermopsin (CYN) is a toxin produced by various cyanobacteria species. Fish can be exposed to this cyanotoxin in their natural environments and in aquaculture ponds, and toxic effects can be derived. The present study investigated the effects of dietary N-acetylcysteine (NAC) on the oxidative stress induced by pure CYN and CYN from lyophilized cells of Aphanizomenon ovalisporum in tilapia (Oreochromis niloticus). Fish were pretreated with 0,22, and 45 mg NAC/fish/d for a week, and on day seven, they received a single dose of 200 μg/kg CYN and were killed after 24 h. Oxidative biomarkers evaluated included lipid peroxidation, protein oxidation, glutathione (GSH)/oxidized glutathione (GSSG) ratio, activity of the enzyme γ-glutamylcysteine synthetase, and activity and gene expression of glutathione-S-transferase and glutathione peroxidase. Results showed that CYN induced oxidative stress as evidenced by the increase of lipid peroxidation and protein oxidation, the decrease in GSH/GSSG, and the alteration of the enzymatic activities assayed. Moreover, exposure to cyanobacterial cells containing CYN induced higher toxic effects in comparison to pure CYN. iV-acetylcysteine supplementation was effective at reducing the toxicity induced by CYN, particularly at the highest dose employed, with a recovery of some of the biomarkers assayed to basal levels. Therefore, NAC can be considered a useful chemoprotectant that reduces hepatic and renal oxidative stress in the prophylaxis and treatment of CYN-related intoxication in fish.
机译：Cylindrospermopsin（CYN）是由多种蓝细菌产生的毒素。鱼可以在其自然环境和水产养殖池塘中暴露于这种氰毒素中，并可能产生毒性作用。本研究调查了日粮N-乙酰半胱氨酸（NAC）对罗非鱼卵圆隐棒菌冻干细胞中纯CYN和CYN诱导的氧化应激的影响。将鱼用0.22和45 mg NAC /鱼/ d预处理一周，在第7天，它们接受200μg/ kg CYN的单剂量，并在24小时后杀死。评估的氧化生物标志物包括脂质过氧化，蛋白质氧化，谷胱甘肽（GSH）/氧化谷胱甘肽（GSSG）比率，γ-谷氨酰半胱氨酸合成酶的活性以及谷胱甘肽-S-转移酶和谷胱甘肽过氧化物酶的活性和基因表达。结果表明，CYN诱导了氧化应激，如脂质过氧化和蛋白质氧化的增加，GSH / GSSG的减少以及酶活性的改变所证明。而且，与纯CYN相比，暴露于包含CYN的蓝细菌细胞诱导更高的毒性作用。 iV-乙酰半胱氨酸补充剂可有效降低CYN诱导的毒性，尤其是在采用最高剂量时，可将一些生物标志物的含量恢复至基础水平。因此，NAC可以被认为是一种有效的化学保护剂，可在预防和治疗鱼类CYN相关中毒中减轻肝脏和肾脏的氧化应激。

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  • 来源
    《Environmental toxicology and chemistry》 |2012年第7期|p.1548-1555|共8页
  • 作者

    Daniel Gutierrez-Praena; Maria Puerto; Ana Isabel Prieto; Angeles Jos; Silvia Pichardo; Vitor Vasconcelos; Ana M. CAMEAN;

  • 作者单位

    Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain;

    Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain;

    Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain;

    Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain;

    Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain;

    Biology Department, Faculty of Sciences, University of Porto, Porto, Portugal Interdisciplinary Center of Marine and Environmental Research-CIIMAR/CIMAR, University of Porto, Porto, Portugal;

    Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cyanobacteria; cylindrospermopsin; tilapia; oxidative stress; N-acetylcysteine;

    机译：蓝细菌环精胺蛋白酶;罗非鱼氧化应激N-乙酰半胱氨酸;

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