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首页> 外文期刊>Environmental toxicology and chemistry >RELATIONSHIPS BETWEEN THE TRANSCRIPTOME AND PHYSIOLOGICAL INDICATORS OF REPRODUCTION IN FEMALE RAINBOW TROUT OVER AN ANNUAL CYCLE
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RELATIONSHIPS BETWEEN THE TRANSCRIPTOME AND PHYSIOLOGICAL INDICATORS OF REPRODUCTION IN FEMALE RAINBOW TROUT OVER AN ANNUAL CYCLE

机译：跨年度女虹鳟繁殖过程中转录谱与生理指标的关系

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Normal transcriptomic patterns along the brain-pituitary-gonad-liver (BPGL) axis should be better characterized if endocrine-disrupting compound-induced changes in gene expression are to be understood. Female rainbow trout were studied over a complete year-long reproductive cycle. Tissue samples from pituitary, ovary, and liver were collected for microarray analysis using the 16K Genomic Research on Atlantic Salmon Project (GRASP) microarray and for quantitative polymerase chain reaction measures of estrogen receptor (ER) isoform messenger RNA (mRNA) levels. Plasma was collected to determine levels of circulating estradiol-17β (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH). As an a priori hypothesis, changes in gene expression were correlated to either circulating levels of E2, FSH, and LH, or ER mRNAs quantified by quantitative polymerase chain reaction. In the liver, most transcriptomic patterns correlated to levels of either E2, LH, or ERs. Fewer ovarian transcripts could be correlated to levels of E2, ERα, or FSH. No significant associations were obvious in the pituitary. As a post hoc hypothesis, changes in transcript abundance were compared with microarray features with known roles in gonadal maturation. Many altered transcripts in the ovary correlated to transcript levels of estradiol 17-beta-dehydrogenase 8 or 17 B HSD12, or to glycoprotein alpha chain 1 or 2. In the pituitary, genes involved with the growth axis (e.g., growth hormone, insulin-related growth factor binding protein) correlated with the most transcripts. These results suggest that transcriptional networks along the BPGL axis may be regulated by factors other than circulating steroid hormones.
机译：如果要理解内分泌干扰性化合物引起的基因表达变化，应更好地表征脑-垂体-性腺-肝（BPGL）轴的正常转录组模式。在整个一年的繁殖周期中研究了雌性虹鳟鱼。收集来自垂体，卵巢和肝脏的组织样本，以使用16K Atlantic Salmon Project基因组研究（GRASP）微阵列进行微阵列分析，以及定量测量雌激素受体（ER）同种型信使RNA（mRNA）水平的聚合酶链反应。收集血浆以确定循环雌二醇-17β（E2），促卵泡激素（FSH）和促黄体生成素（LH）的水平。作为先验假设，基因表达的变化与通过定量聚合酶链反应定量的E2，FSH和LH或ER mRNA的循环水平相关。在肝脏中，大多数转录组模式与E2，LH或ER的水平相关。较少的卵巢转录本可能与E2，ERα或FSH的水平相关。垂体无明显关联。作为事后假设，将转录本丰度的变化与已知在性腺成熟中起作用的微阵列特征进行比较。卵巢中许多改变的转录物与雌二醇17-β-脱氢酶8或17 B HSD12的转录物水平或糖蛋白α链1或2相关。在垂体中，与生长轴有关的基因（例如，生长激素，胰岛素-相关的生长因子结合蛋白）与转录本最多相关。这些结果表明，沿着BPGL轴的转录网络可能受循环类固醇激素以外的因素调节。

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