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首页> 外文期刊>Environmental toxicology and chemistry >Microarray Analysis Of Polychlorinated Biphenyl Mixture-inducedchanges In Gene Expression Among Atlantic Tomcod Populationsrndisplaying Differential Sensitivity To Halogenatedrnaromatic Hydrocarbons
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Microarray Analysis Of Polychlorinated Biphenyl Mixture-inducedchanges In Gene Expression Among Atlantic Tomcod Populationsrndisplaying Differential Sensitivity To Halogenatedrnaromatic Hydrocarbons

机译：微阵列分析多氯联苯混合物诱导表达差异对卤代芳烃的大西洋Tomcod种群之间基因表达的变化。

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Several populations of fishes inhabiting contaminated Atlantic Coast estuaries exhibit resistance to early life-stage (ELS) toxicities induced by halogenated aromatic hydrocarbons such as coplanar polychlorinated biphenyls (PCBs). These toxicities include mortality, circulatory failure, edema, and craniofacial malformations. The mechanisms behind resistance to halogenated aromatic hydrocarbon toxicity in these populations are unknown. First and second generation Atlantic tomcod Microgadus tomcod embryos derived from the Hudson River ([HR]; New York, USA) population are highly resistant to PCB-induced cytochrome P4501A (CYP1A) expression and ELS toxicity when compared to embryos of Miramichi River ([MR]; New Brunswick, Canada) and Shinnecock Bay ([SB]; New York, USA) origin. The present study sought to identify novel genes involved in population differences in response to PCB exposure using custom microarrays. Microarray probes consisted of unsequenced inserts of randomly picked clones from a tomcod cardiac cDNA library. Tomcod embryos from three populations (HR, MR, and SB) were exposed to two doses of an environmentally relevant mixture of coplanar PCBs and screened for dose- and population-specific patterns of gene expression. Clones displaying significant differences between populations exposed to the high dose of PCBs were identified by DNA sequencing. Of the 28 identified nonribosomal protein clones, none displayed expression patterns highly similar to CYP1A (altered in MR and SB, but not in HR). However, several transcripts representing biomarkers of cardiomyopathy in mammals (cardiac troponin T2, cathepsin L, and atrial natriuretic peptide) were differentially altered among the three tomcod populations by PCBs. Although the present study did not identify any novel genes associated with PCB resistance in tomcod, several potential molecular biomarkers of PCB exposure were revealed.
机译：居住在受污染的大西洋海岸河口的几种鱼类对由共面多氯联苯（PCB）等卤代芳烃引起的生命早期（ELS）毒性具有抵抗力。这些毒性包括死亡率，循环衰竭，水肿和颅面畸形。这些人群对卤代芳烃毒性具有抗性的背后机制尚不清楚。与Miramichi River的胚胎相比，源自哈德逊河（[HR]；纽约，美国）的第一代和第二代大西洋杂种Microgadus杂种胚胎对PCB诱导的细胞色素P4501A（CYP1A）表达和ELS毒性具有高度抗性。 MR]；加拿大新不伦瑞克省和Shinnecock湾（[SB]；美国纽约）起源。本研究试图使用定制的微阵列鉴定与PCB暴露相关的种群差异的新基因。微阵列探针由来自tomcod心脏cDNA文库的随机挑选的克隆的未测序插入物组成。将来自三个种群（HR，MR和SB）的Tomcod胚胎暴露于两剂共平面PCB的环境相关混合物中，并筛选基因表达的剂量和种群特异性模式。通过DNA测序鉴定了在暴露于高剂量PCBs的群体之间显示出显着差异的克隆。在鉴定出的28个非核糖体蛋白克隆中，没有一个显示与CYP1A高度相似的表达模式（在MR和SB中有所改变，但在HR中则没有）。但是，在三种tomcod种群中，代表PCB的几种代表哺乳动物心肌病生物标志物的转录物（心肌肌钙蛋白T2，组织蛋白酶L和心钠素）都有差异。尽管本研究没有发现与线虫中PCB抗性相关的任何新基因，但揭示了PCB暴露的几种潜在分子生物标记。

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