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首页> 外文期刊>Environmental toxicology and chemistry >GENE EXPRESSION ANALYSIS IN ZEBRAFISH EMBRYOS: A POTENTIAL APPROACH TO PREDICT EFFECT CONCENTRATIONS IN THE FISH EARLY LIFE STAGE TEST
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GENE EXPRESSION ANALYSIS IN ZEBRAFISH EMBRYOS: A POTENTIAL APPROACH TO PREDICT EFFECT CONCENTRATIONS IN THE FISH EARLY LIFE STAGE TEST

机译：斑马鱼胚胎中的基因表达分析：一种预测鱼早期生命期试验中效应浓度的潜在方法

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Based on the hypothesis that analysis of gene expression could be used to predict chronic fish toxicity, the zebrafish (Danio rerio) embryo test (DarT), developed as a replacement method for the acute fish test, was expanded to a gene expression D. rerio embryo test (Gene-DarT). The effects of 14 substances on lethal and sublethal endpoints of the DarT and on expression of potential marker genes were investigated: the aryl hydrocarbon receptor 2, cytochrome P450 1A (cypla), heat shock protein 70, fizzy-related protein 1, the transcription factors v-maf musculoaponeurotic fibrosarcoma oncogene family protein g (avian) 1 and NF-E2-p45-related factor, and heme oxygenase 1 (hmox1). After exposure of zebrafish embryos for 48 h, differential gene expression was evaluated using reverse transcriptase-polymerase chain reaction, gel electrophoresis, and densitometric analysis of the gels. All tested compounds significantly affected the expression of at least one potential marker gene, with cypla and hmox1 being most sensitive. Lowest-observed-effect concentrations (LOECs) for gene expression were below concentrations resulting in 10% lethal effects in the DarT. For 10 (3,4- and 3,5-dichloroaniline, 1,4-dichlorobenzene, 2,4-dinitrophenol, atrazine, parathion-ethyl, chlorotoluron, genistein, 4-nitroquinoline-l-oxide, and cadmium) out of the 14 tested substances, LOEC values derived with the Gene-DαrT differ by a factor of less than 10 from LOEC values offish early life stage tests with zebrafish. For pentachloroaniline and pentachlorobenzene, the Gene-DarT showed a 23- and 153-fold higher sensitivity, respectively, while for lindane, it showed a 13-fold lower sensitivity. For ivermectin, the Gene-DarT was by a factor of more than 1,000 less sensitive than the acute fish test. The results of the present study indicate that gene expression analysis in zebrafish embryos could principally be used to predict effect concentrations in the fish early life stage test.
机译：基于可以通过基因表达分析来预测慢性鱼类毒性的假设，将斑马鱼（Danio rerio）胚胎试验（DarT）作为急性鱼试验的替代方法开发，扩展到了基因表达D. rerio胚胎测试（Gene-DarT）。研究了14种物质对DarT致死和亚致死终点以及潜在标志物基因表达的影响：芳烃受体2，细胞色素P450 1A（cypla），热休克蛋白70，泡沫相关蛋白1，转录因子v-maf肌腱膜纤维肉瘤癌基因家族蛋白g（禽）1和NF-E2-p45相关因子和血红素加氧酶1（hmox1）。斑马鱼胚胎暴露48小时后，使用逆转录酶-聚合酶链反应，凝胶电泳和凝胶密度分析法评估差异基因表达。所有测试的化合物均显着影响至少一种潜在标记基因的表达，其中cypla和hmox1最敏感。基因表达的最低观测效应浓度（LOEC）低于在DarT中导致10％致死作用的浓度。对于其中的10种（3,4-和3,5-二氯苯胺，1,4-二氯苯，2,4-二硝基苯酚，at去津，对硫磷-乙基，氯甲苯隆，染料木黄酮，4-硝基喹啉-1-氧化物和镉）在14种被测物质中，通过Gene-DαrT得出的LOEC值与斑马鱼鱼类早期生命期测试的LOEC值相差不到10倍。对于五氯苯胺和五氯苯，Gene-DarT分别显示高23倍和153倍的敏感性，而对于林丹，它显示低13倍的敏感性。对于伊维菌素而言，Gene-DarT的敏感性比急性鱼类测试低1,000倍。本研究的结果表明，斑马鱼胚胎中的基因表达分析可主要用于预测鱼生命早期测试中的效应浓度。

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