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首页> 外文期刊>Environmental toxicology and chemistry >A NONINVASIVE TEST OF EXPOSITION TO TOXICANTS: QUANTITATIVE ANALYSIS OF CYTOCHROME P4501A EXPRESSION IN FISH SCALES
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A NONINVASIVE TEST OF EXPOSITION TO TOXICANTS: QUANTITATIVE ANALYSIS OF CYTOCHROME P4501A EXPRESSION IN FISH SCALES

机译：暴露于毒素的无创试验：鱼鳞中细胞色素P4501A表达的定量分析

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摘要

Elevatec expression of cytochrome P4501A (CYP1A) is an established biomarker for exposition to a wide range of toxicants, particularly for dioxin and structurally similar compounds. Expression of CYP1A usually is analyzed in internal organs, which involves dissection of the specimen. To avoid unnecessary animal killing, we present here an alternative method based on the monitoring of CYP1A expression in fish scales. Using β-naphthoflavone (BNF; 50 mg/kg body wt, intraperitoneal injection) as inducer in goldfish (Carassius auratus), we monitored levels of CYP1A mRNA both in scales and liver of treated and control specimens. Treatment with BNF resulted in a similar induction of CYP1A gene in both tissues, although scales responded faster (at 8 h after treatment) than liver (between 24 and 48 h). The scale-based test has the unique advantage of allowing sequential testing in the same specimen, which facilitates analysis of the time course of CYP1A induction and allows the study of individual variability. The method implies minimal suffering of the animals, because it only requires removal of a moderate (n = 1-3) number of scales for each time point. This nondestructive, fast, and relatively inexpensive test for toxic exposure therefore is suitable for environmental monitoring and food safety control programs in which specimen preservation is required.
机译：Elevatec的细胞色素P4501A（CYP1A）表达是一种确定的生物标记，可暴露于多种有毒物质，尤其是二恶英和结构相似的化合物。 CYP1A的表达通常在内部器官中进行分析，这涉及标本的解剖。为避免不必要的动物杀害，我们在此提出了一种基于监测鱼鳞中CYP1A表达的替代方法。使用β-萘黄酮（BNF； 50 mg / kg体重，腹膜内注射）作为金鱼（Car鱼）的诱导剂，我们在治疗和对照样本的鳞片和肝脏中监测CYP1A mRNA的水平。 BNF的治疗在两个组织中均引起了类似的CYP1A基因诱导，尽管鳞片（治疗后8小时）的反应快于肝脏（24至48小时）。基于量表的测试具有允许在同一样本中进行顺序测试的独特优势，这有利于分析CYP1A诱导的时间过程并允许研究个体变异性。该方法意味着对动物的痛苦最小，因为它只需要在每个时间点去除中等数量（n = 1-3）的鳞片即可。因此，这种无毒，快速且相对廉价的毒性测试适合于需要样品保存的环境监测和食品安全控制程序。

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  • 来源
    《Environmental toxicology and chemistry》 |2007年第10期|2179-2186|共8页
  • 作者

    Laia Quiros; Demetrio Raldua; Anna Navarro; Marta Casado; DamiA Barcelo; Benjamin Pina;

  • 作者单位

    Department of Molecular Biology, Institute of Molecular Biology of Barcelona, Consejo Superior de Investigaciones Cientificas (IBMB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain;

    Laboratory of Environmental Toxicology, Universitat Politecnica de Catalunya, CN 150, Km. 14.5 (Institut Politecnic Campus Terrassa), 08220 Terrassa, Spain;

    Department of Molecular Biology, Institute of Molecular Biology of Barcelona, Consejo Superior de Investigaciones Cientificas (IBMB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain;

    Department of Molecular Biology, Institute of Molecular Biology of Barcelona, Consejo Superior de Investigaciones Cientificas (IBMB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain;

    Department of Environmental Chemistry, Institute of Chemical and Environmental Research, Consejo Superior de Investigaciones Cientificas (IIQAB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain;

    Department of Molecular Biology, Institute of Molecular Biology of Barcelona, Consejo Superior de Investigaciones Cientificas (IBMB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    quantitative real-time polymerase chain reaction; fish ecotoxicology; genetic biomarkers; environmental biomonitoring; aryl hydrocarbon receptor;

    机译：定量实时聚合酶链反应;鱼类生态毒理学;遗传生物标记;环境生物监测;芳烃受体;

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