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Gender Difference In The Composition Of Fecal Flora Inlaboratory Mice, As Detected By Denaturing Gradientrngel Electrophoresis (dgge)

机译:通过变性梯度凝胶电泳(dgge)检测的粪便菌群实验室小鼠的性别差异

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The difference between genders in the composition of mouse fecal flora was examined. A polymerase chain reaction followed by denaturing gradient gel electrophoresis (DGGE) were performed on the V6-V8 regions of bacterial 16S rDNA obtained from fecal samples at 0, 1, 2, 3, 4, and 8 weeks after the introduction of mice into the laboratory from a mouse farm. Cluster analysis and non-metric multidimensional scaling (NMDS) were then performed. Male and female mice were distributed on opposite sides of the origin of the plane in NMDS at weeks 0, 2, 3, and 8. These results suggest a gender difference in the composition of intestinal flora in mice.
机译:检查了小鼠粪便菌群组成中的性别差异。在将小鼠引入小鼠的0、1、2、3、4和8周后,对从粪便样品获得的细菌16S rDNA的V6-V8区进行聚合酶链反应,然后进行变性梯度凝胶电泳(DGGE)。老鼠场的实验室。然后进行聚类分析和非度量多维标度(NMDS)。在第0、2、3和8周,雄性和雌性小鼠分布在NMDS中平面起点的相对侧。这些结果表明,小鼠肠道菌群组成存在性别差异。

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