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An involvement of mouse Spesp 1 in sperm-egg fusion in vitro

机译：小鼠Spesp 1参与体外精卵融合

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Mammalian fertilization is a multistep process that culminates in the fusion of the sperm and oocyte plasma membranes. The factors involved in this important membrane fusion have long been sought. By producing Izumo deficient mouse line, we identified IZUMO protein on sperm as indispensable in sperm-egg fusion. It is widely accepted that the equatorial segment of the acrosome-reacted sperm is important in initiating fusion with the egg membrane during fertilization. IZUMO appears in this region only after the acrosome reaction, but the distribution is not limited to the equatorial segment. This led us to speculate that there must be a protein localizing only in the equatorial segment and functioning in the fertilization process. In this context, SPESP1 (sperm equatorial segment protein 1) that has been localized in the equatorial segment of ejaculated human sperm caught our attention. To examine the role of mouse SPESP1 in the fertilization process, we produced Spespl gene-disrupted mice by homologous recombination. Spespl(-/-) mutant mice were healthy and showed no overt developmental abnormalities. Moreover, differing from our expectation, they showed normal fertilizing ability in both males and females in vivo. However, interestingly, when the fertilizing ability of Spespl(-/-) sperm was examined in in vitro fertilization system, both Spespl(+/-) and (-/-) sperm were found to be less competent in fertilizing eggs compared to wild-type sperm. Further investigation to clarify the role of SPESP1 in fertilization will be beneficial to elucidating the mechanism of fertilization.
机译：哺乳动物的受精是一个多步骤的过程，最终使精子和卵母细胞质膜融合。长期以来一直在寻找与这种重要的膜融合有关的因素。通过产生Izumo缺陷小鼠品系，我们鉴定出精子上的IZUMO蛋白是精卵融合中必不可少的。人们普遍认为，顶体反应的精子的赤道部分在受精过程中与卵膜的融合中起重要作用。 IZUMO仅在顶体反应后出现在该区域中，但是分布不限于赤道片段。这使我们推测必须存在仅在赤道部分定位并在受精过程中起作用的蛋白质。在这种情况下，已经定位在射精后的人类精子的赤道部分中的SPESP1（精子赤道部分蛋白1）引起了我们的注意。为了检查小鼠SPESP1在受精过程中的作用，我们通过同源重组制备了Spespl基因破坏的小鼠。 Spespl（-/-）突变小鼠健康，没有明显的发育异常。此外，与我们的预期不同，它们在体内的雄性和雌性中均显示出正常的受精能力。但是，有趣的是，当在体外受精系统中检查Spespl（-/-）精子的受精能力时，与野生相比，Spespl（+/-）和（-/-）精子的受精能力均较低。型精子。进一步研究以阐明SPESP1在受精中的作用将有助于阐明受精机理。

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