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首页> 外文期刊>Experimental Heat Transfer >ELECTROPHORESIS POLY(DIMETHYLSILOXANE)/GLASS CHIPS WITH INTEGRATED ACTIVE COOLING FOR QUANTIFICATION OF AMINO ACIDS
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ELECTROPHORESIS POLY(DIMETHYLSILOXANE)/GLASS CHIPS WITH INTEGRATED ACTIVE COOLING FOR QUANTIFICATION OF AMINO ACIDS

机译：集成活性冷却的电泳聚（二甲基硅氧烷）/玻璃碎片用于氨基酸的定量

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摘要

The objective of this work was to develop and characterize a poly(dimethylsiloxane) device with an integrated active cooling function able to carry out capillary electrophoresis separations. Polymer-based microdevices are indispensable to recent advances in biomedical analysis. In particular, they have been applied to many microfluidic platforms owing to their low cost, ease of fabrication, and versatility in preparing complex microstructures. However, when applied to capillary electrophoresis separations, polymer microfluidic structures present an inherent disadvantage compared to glass and Si structures; they have a lower thermal conductivity than glass and Si. Although miniaturized devices allow operation at high electric fields, they face separation efficiency limitations due to Joule heating. There is, therefore, a strong need of developing capillary electrophoresis microfluidic structures with active cooling in order to operate at a higher electric field and potentially increase separation efficiency in these microdevices. A poly(dimethylsiloxane)/glass hybrid microfluidic capillary electrophoresis system is presented, where Joule heating was minimized by using an integrated active cooling function. Two poly(dimethylsiloxane) slabs with embedded microfluidic structures were irreversibly sealed on both sides of a thin glass slide. The top poly(dimethylsiloxane) slab was used to carry out capillary electrophoresis separations, whereas the bottom poly(dimethylsiloxane) slab was employed to cooldown the buffer solution used during the capillary electrophoresis separation. As demonstrated on current versus voltage plots and on capillary electrophoresis electropherograms, capillary electrophoresis separation was able to be operated at a higher electric field when using the cooling function. The cooling rate was adjustable by varying the flow rate and the initial temperature of the liquid flowing in the cooling microfluidic structure.
机译：这项工作的目的是开发和表征一种具有集成主动冷却功能的聚二甲基硅氧烷器件，该器件能够进行毛细管电泳分离。基于聚合物的微型设备对于生物医学分析的最新进展必不可少。特别地，由于它们的低成本，易于制造以及在制备复杂的微结构中的多功能性，它们已被应用于许多微流体平台。然而，当用于毛细管电泳分离时，与玻璃和硅结构相比，聚合物微流体结构存在固有的缺点。它们的热导率比玻璃和硅低。尽管小型化的设备允许在高电场下运行，但由于焦耳热，它们面临着分离效率的限制。因此，迫切需要开发具有主动冷却功能的毛细管电泳微流体结构，以便在更高的电场下工作并潜在地提高这些微型设备中的分离效率。提出了一种聚（二甲基硅氧烷）/玻璃混合微流体毛细管电泳系统，其中通过使用集成的主动冷却功能将焦耳热降至最低。将两个嵌入微流体结构的聚（二甲基硅氧烷）平板不可逆地密封在薄玻璃片的两侧。顶部聚二甲基硅氧烷平板用于进行毛细管电泳分离，而底部聚二甲基硅氧烷平板用于冷却在毛细管电泳分离过程中使用的缓冲液。如电流对电压图和毛细管电泳电泳图所示，当使用冷却功能时，毛细管电泳分离能够在更高的电场下进行。通过改变冷却微流体结构中流动的液体的流速和初始温度，可以调节冷却速率。

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  • 来源
    《Experimental Heat Transfer》 |2010年第1期|63-72|共10页
  • 作者

    L. Renaud; O. Yassine; P. Kleimann; A.-L. Deman; J.-F. Chateaux; P. Morin; N. Ouaini; R. Ferrigno;

  • 作者单位

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

    Faculte des Sciences et de Genie Informatique, Universite Saint Esprit de Kaslik, Jounieh, Liban;

    INL Institut des Nanotechnologies de Lyon, Villeurbanne, France CNRS UMR5270, Villeurbanne, France Universite de Lyon, Lyon, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Joule heating; capillary electrophoresis separation; temperature measurements; microfluidics; lab-on-chip;

    机译：焦耳加热;毛细管电泳分离温度测量;微流体芯片实验室;

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