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首页> 外文期刊>Fish Physiology and Biochemistry >Identification and expression of Smads associated with TGF-β/activinodal signaling pathways in the rainbow trout (Oncorhynchus mykiss)
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Identification and expression of Smads associated with TGF-β/activinodal signaling pathways in the rainbow trout (Oncorhynchus mykiss)

机译：与虹鳟鱼（Oncorhynchus mykiss）中TGF-β/激活素/节点信号通路相关的Smads的鉴定和表达

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摘要

The Smad proteins are essential components of the TGF-β/activinodal family signaling pathway. We report the identification and expression of transcripts representing three receptor Smads (Smad2a, Smad2b, and Smad3), two common Smads (Smad4a and Smad4b), and one inhibitory Smad (Smad7). Phylogenetic analysis suggests this gene family evolved through the combination of ancient and more recent salmonid genome duplication events. Tissue distribution, embryonic expression, and expression in growth hormone (GH) treated fish were assessed by reverse transcription PCR or qPCR. All six Smad transcripts were ubiquitously expressed in adult tissues. We observed the highest expression of the receptor Smads in unfertilized eggs, generally decreasing during early embryonic development and slightly increasing around 11 days post-fertilization (dpf). Smad7 expression was low for most of embryonic development, with a dramatic increase at the onset of muscle development (6 dpf), and at hatch (24 dpf). Smad4 expression was low during early embryonic development and increased after 14 dpf. The increased expression of Smad4 and Smad7 during late embryonic development may indicate modulation of gene expression by GH axis, which initiates activity during late embryonic development. These data were supported by the modulation of these Smads in the gill filament, stomach, and muscle following a GH treatment. Additionally, these changes are concurrent with the modulation of expression of TGF-β family members. Most significantly, the increased expression of Smad7 in the muscle is simultaneous with increased expression of MSTN1A and not MSTN1B during both embryonic development and following GH treatment. These data indicate a promyogenic role for Smad7 as previously identified in other non-fish species.
机译：Smad蛋白是TGF-β/激活素/节点家族信号通路的重要组成部分。我们报告鉴定和表达的成绩单代表三个受体Smads（Smad2a，Smad2b和Smad3），两个常见的Smads（Smad4a和Smad4b）和一个抑制性Smad（Smad7）。系统发育分析表明，该基因家族是通过结合古代和最近的鲑鱼基因组复制事件而进化而来的。通过逆转录PCR或qPCR评估组织分布，胚胎表达和生长激素（GH）处理鱼中的表达。所有六个Smad转录本均在成人组织中普遍表达。我们观察到未受精卵中Smads受体的最高表达，通常在早期胚胎发育过程中降低，而在受精后11天左右（dpf）则略有升高。在大多数胚胎发育中，Smad7表达均较低，在肌肉发育开始时（6 dpf）和孵化时（24 dpf）都有显着增加。 Smad4表达在早期胚胎发育过程中较低，并在14 dpf后增加。 Smad4和Smad7在晚期胚胎发育过程中表达的增加可能表明基因表达受到GH轴的调节，从而启动了晚期胚胎发育过程中的活性。在GH治疗后，这些Smads在filament丝，胃和肌肉中的调节支持了这些数据。另外，这些变化与TGF-β家族成员表达的调节同时发生。最重要的是，在胚胎发育过程中和GH治疗后，肌肉中Sma​​d7表达的增加与MSTN1A而不是MSTN1B的表达增加同时发生。这些数据表明，先前在其他非鱼类物种中发现的Smad7具有促肌生作用。

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  • 来源
    《Fish Physiology and Biochemistry》 |2012年第5期|p.1233-1244|共12页
  • 作者

    Scott A. Gahr; Gregory M. Weber; Caird E. Rexroad III;

  • 作者单位

    Biology Department, St. Vincent College, 300 Fraser Purchase Rd., Latrobe, PA, 15650, USA;

    United States Department of Agriculture, Agriculture Research Service, National Center for Cool and Cold Water Aquaculture, Kearneysville, WV, 25430, USA;

    United States Department of Agriculture, Agriculture Research Service, National Center for Cool and Cold Water Aquaculture, Kearneysville, WV, 25430, USA;

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  • 正文语种 eng
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  • 关键词

    Embryo; Growth hormone; Trout; Fish; Smad; TGF-β; Activin; Signaling; Myostatin;

    机译：胚胎;生长激素;鳟鱼;鱼;Smad;TGF-β;激活素;信号传导;肌生长抑制素;

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