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首页> 外文期刊>Food additives & contaminants >Simultaneous determination of multi-class antibiotics and steroid hormones drugs in livestock and poultry faeces using liquid chromatography-quadrupole time-of-flight mass spectrometry
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Simultaneous determination of multi-class antibiotics and steroid hormones drugs in livestock and poultry faeces using liquid chromatography-quadrupole time-of-flight mass spectrometry

机译:使用液相色谱 - 四极针对飞行时间质谱法同时测定畜禽粪便中的多级抗生素和类固醇激素药物

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摘要

A method for simultaneous determination of multi-class antibiotics and steroid hormone analysis in faeces of livestock and poultry was developed using liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF MS). An in-house database was built for 156 detected drugs using Personal Compound Database Library software (PCDL) including compound name, mono-isotopic mass, chemical formula, RT, chemical structure and three CID MS/MS spectra. The linearity result showed that all the drugs exhibited good linearity with determination coefficients (R~2) higher than 0.99. The drug recoveries and their RSDs for all three faeces samples (pig, cattle and chicken) were tested and 81, 96 and 92 drugs were chosen for analysis in pig, cattle and chicken faeces, respectively. Further validation showed that 73 veterinary drugs in all three kinds of faeces samples can be quantified in one analytical run. This work shows that qualitative and quantitative analysis using LC-QTOF MS represents a simple, sensitive, low-cost and high-throughput methodology in routine laboratory analyses.
机译:采用液相色谱 - 四极杆飞行时间质谱(LC-QTOF MS),开发了一种同时测定畜禽和家禽粪便和家禽的多级抗生素和类固醇激素分析的方法。使用个人化合物数据库库软件(PCDL)为156个检测到的药物构建了内部数据库,包括复合名称,单同位素质量,化学式,室温,化学结构和三种CID MS / MS光谱。线性度结果表明,所有药物表现出良好的线性度,测定系数(R〜2)高于0.99。测试所有三种粪便样品(猪,牛和鸡)的药物回收率及其RSDS分别选择81,96和92种药物分析猪,牛和鸡粪便。进一步的验证表明,所有三种粪便样品中的73名兽药都可以在一个分析运行中量化。这项工作表明,使用LC-QTOF MS的定性和定量分析代表了常规实验室分析中简单,灵敏,低成本和高通量的方法。

著录项

  • 来源
    《Food additives & contaminants》 |2020年第9期|1467-1480|共14页
  • 作者单位

    Key Laboratory of Agro-food Safety and Quality Ministry of Agriculture and Rural Affairs Institute of Quality Standard & Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences No. 12 Zhongguancun South Street Haidian District Beijing 100081 China College of Food Science and Nutritional Engineering China Agricultural University Beijing China;

    Key Laboratory of Agro-food Safety and Quality Ministry of Agriculture and Rural Affairs Institute of Quality Standard & Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences No. 12 Zhongguancun South Street Haidian District Beijing 100081 China;

    Key Laboratory of Agro-food Safety and Quality Ministry of Agriculture and Rural Affairs Institute of Quality Standard & Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences No. 12 Zhongguancun South Street Haidian District Beijing 100081 China;

    Key Laboratory of Agro-food Safety and Quality Ministry of Agriculture and Rural Affairs Institute of Quality Standard & Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences No. 12 Zhongguancun South Street Haidian District Beijing 100081 China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Time-of-flight mass spectrometry; livestock and poultry faeces; quantitative analysis; antibiotics; steroid hormones;

    机译:飞行时间质谱;牲畜和家禽粪便;定量分析;抗生素;类固醇荷尔蒙;

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